Sequence of rat intestinal vitamin D-dependent calcium-binding protein derived from a cDNA clone. Evolutionary implications

C. Desplan, O. Heidmann, J. W. Lillie, C. Auffray, M. Thomasset

Research output: Contribution to journalArticle

Abstract

We have recently reported molecular cloning of the cDNA synthesized from rat duodenal mRNA-encoding intestinal calcium-binding protein (ICaBP), a vitamin D3-induced protein (Desplan, C., Thomasset, M., and Moukhtar, M.S. (1983) J. Biol. Chem. 258, 2762-2765). Nucleotide sequence analysis of the longest cDNA insert (375 base pairs) permitted the assignment of 207 nucleotides of the coding region and 104 nucleotides of the entire 3'-noncoding region of the mRNA. Although the derived amino acid sequence for rat ICaBP differed from the bovine and porcine sequences by 16 and 14 residues, respectively, all the residues of each calcium-binding site met the proposed requirements of the 'EF hand' theory. In contrast, several differences found in the linker regions might explain the absence of cross-immunoreactivity between rat and porcine ICaBPs. Analysis of nucleotide sequence homologies between the coding and noncoding regions showed that the region coding for the two calcium-binding sites (I and II) was immediately followed in the noncoding region by a sequence very similar to the sequence coding for site I. This suggests that rat ICaBP mRNA contains the remains of an untranslated calcium-binding site III-like structure and that low M(r) ICaBP could result in early termination of the translation of a larger molecule containing four sites.

Original languageEnglish (US)
Pages (from-to)13502-13505
Number of pages4
JournalJournal of Biological Chemistry
Volume258
Issue number22
StatePublished - 1983

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S100 Calcium Binding Protein G
Calcium-Binding Proteins
Rats
Nucleotides
Complementary DNA
Clone Cells
Binding Sites
Calcium
Messenger RNA
Swine
EF Hand Motifs
Cholecalciferol
Cloning
Molecular Cloning
Sequence Homology
Base Pairing
Sequence Analysis
Amino Acid Sequence
Amino Acids
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sequence of rat intestinal vitamin D-dependent calcium-binding protein derived from a cDNA clone. Evolutionary implications. / Desplan, C.; Heidmann, O.; Lillie, J. W.; Auffray, C.; Thomasset, M.

In: Journal of Biological Chemistry, Vol. 258, No. 22, 1983, p. 13502-13505.

Research output: Contribution to journalArticle

Desplan, C. ; Heidmann, O. ; Lillie, J. W. ; Auffray, C. ; Thomasset, M. / Sequence of rat intestinal vitamin D-dependent calcium-binding protein derived from a cDNA clone. Evolutionary implications. In: Journal of Biological Chemistry. 1983 ; Vol. 258, No. 22. pp. 13502-13505.
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