Seq-Well

portable, low-cost RNA sequencing of single cells at high throughput

Todd M. Gierahn, Marc H. Wadsworth, Travis K. Hughes, Bryan D. Bryson, Andrew Butler, Rahul Satija, Sarah Fortune, J. Christopher Love, Alex K. Shalek

Research output: Contribution to journalArticle

Abstract

Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We use Seq-Well to profile thousands of primary human macrophages exposed to Mycobacterium tuberculosis.

Original languageEnglish (US)
JournalNature Methods
DOIs
StateAccepted/In press - Feb 13 2017

Fingerprint

RNA Sequence Analysis
Throughput
RNA
Costs and Cost Analysis
Macrophages
Costs
Membranes
Messenger RNA
Mycobacterium tuberculosis

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Gierahn, T. M., Wadsworth, M. H., Hughes, T. K., Bryson, B. D., Butler, A., Satija, R., ... Shalek, A. K. (Accepted/In press). Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput. Nature Methods. https://doi.org/10.1038/nmeth.4179

Seq-Well : portable, low-cost RNA sequencing of single cells at high throughput. / Gierahn, Todd M.; Wadsworth, Marc H.; Hughes, Travis K.; Bryson, Bryan D.; Butler, Andrew; Satija, Rahul; Fortune, Sarah; Love, J. Christopher; Shalek, Alex K.

In: Nature Methods, 13.02.2017.

Research output: Contribution to journalArticle

Gierahn, TM, Wadsworth, MH, Hughes, TK, Bryson, BD, Butler, A, Satija, R, Fortune, S, Love, JC & Shalek, AK 2017, 'Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput', Nature Methods. https://doi.org/10.1038/nmeth.4179
Gierahn, Todd M. ; Wadsworth, Marc H. ; Hughes, Travis K. ; Bryson, Bryan D. ; Butler, Andrew ; Satija, Rahul ; Fortune, Sarah ; Love, J. Christopher ; Shalek, Alex K. / Seq-Well : portable, low-cost RNA sequencing of single cells at high throughput. In: Nature Methods. 2017.
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