Separation of DNA replication from the assembly of break-competent meiotic chromosomes

Hannah G. Blitzblau, Clara S. Chan, Andreas Hochwagen, Stephen P. Bell

Research output: Contribution to journalArticle

Abstract

The meiotic cell division reduces the chromosome number from diploid to haploid to form gametes for sexual reproduction. Although much progress has been made in understanding meiotic recombination and the two meiotic divisions, the processes leading up to recombination, including the prolonged pre-meiotic S phase (meiS) and the assembly of meiotic chromosome axes, remain poorly defined. We have used genome-wide approaches in Saccharomyces cerevisiae to measure the kinetics of pre-meiotic DNA replication and to investigate the interdependencies between replication and axis formation. We found that replication initiation was delayed for a large number of origins in meiS compared to mitosis and that meiotic cells were far more sensitive to replication inhibition, most likely due to the starvation conditions required for meiotic induction. Moreover, replication initiation was delayed even in the absence of chromosome axes, indicating replication timing is independent of the process of axis assembly. Finally, we found that cells were able to install axis components and initiate recombination on unreplicated DNA. Thus, although pre-meiotic DNA replication and meiotic chromosome axis formation occur concurrently, they are not strictly coupled. The functional separation of these processes reveals a modular method of building meiotic chromosomes and predicts that any crosstalk between these modules must occur through superimposed regulatory mechanisms.

Original languageEnglish (US)
Article numbere1002643
JournalPLoS Genetics
Volume8
Issue number5
DOIs
StatePublished - May 2012

Fingerprint

DNA replication
DNA Replication
chromosome
Chromosomes
chromosomes
DNA
Genetic Recombination
recombination
interphase
S Phase
sexual reproduction
haploidy
mitosis
chromosome number
Haploidy
starvation
cell division
gamete
Saccharomyces cerevisiae
germ cells

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Separation of DNA replication from the assembly of break-competent meiotic chromosomes. / Blitzblau, Hannah G.; Chan, Clara S.; Hochwagen, Andreas; Bell, Stephen P.

In: PLoS Genetics, Vol. 8, No. 5, e1002643, 05.2012.

Research output: Contribution to journalArticle

Blitzblau, Hannah G. ; Chan, Clara S. ; Hochwagen, Andreas ; Bell, Stephen P. / Separation of DNA replication from the assembly of break-competent meiotic chromosomes. In: PLoS Genetics. 2012 ; Vol. 8, No. 5.
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