Separation and properties of rabbit buccal mucosal wound hyaluronidase.

Charles Bertolami, R. H. Day, D. G. Ellis

Research output: Contribution to journalArticle

Abstract

This study establishes the existence of a mammalian buccal mucosal wound hyaluronidase (hyaluronate 4-glycohydrolase; EC 3.2.1.35) having properties distinct from those of the endogenous lysosomal hyaluronidase of normal (uninjured) buccal mucosa. A time-dependent change in hyaluronidase activity was measured, with the highest specific activity occurring on post-wound day 4 (7.7 +/- 1.3 units/mg protein), followed by consecutive decreases until activity was no longer discernible by day 21. Mucosal wound hyaluronidase closely resembled a previously described integumentary wound endoglycosidase in terms of a high pH optimum (5.0-6.0), distinct (but non-exclusive) substrate preference for hyaluronic acid, and ability to generate saturated depolymerization products by an endoglycosidic hydrolysis.

Original languageEnglish (US)
Pages (from-to)939-944
Number of pages6
JournalJournal of Dental Research
Volume65
Issue number6
StatePublished - Jun 1986

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Hyaluronoglucosaminidase
Cheek
Rabbits
Glycoside Hydrolases
Wounds and Injuries
Mouth Mucosa
Hyaluronic Acid
Hydrolysis
Proteins

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Separation and properties of rabbit buccal mucosal wound hyaluronidase. / Bertolami, Charles; Day, R. H.; Ellis, D. G.

In: Journal of Dental Research, Vol. 65, No. 6, 06.1986, p. 939-944.

Research output: Contribution to journalArticle

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