Separate loci underlie resistance to root infection and leaf scorch during soybean sudden death syndrome

S. Kazi, J. Shultz, J. Afzal, J. Johnson, V. N. Njiti, D. A. Lightfoot

Research output: Contribution to journalArticle

Abstract

Soybean [Glycine max (L.) Merr.] cultivars show differences in their resistance to both the leaf scorch and root rot of sudden death syndrome (SDS). The syndrome is caused by root colonization by Fusarium virguliforme (ex. F. solani f. sp. glycines). Root susceptibility combined with reduced leaf scorch resistance has been associated with resistance to Heterodera glycines HG Type 1.3.6.7 (race 14) of the soybean cyst nematode (SCN). In contrast, the rhg1 locus underlying resistance to Hg Type 0 was found clustered with three loci for resistance to SDS leaf scorch and one for root infection. The aims of this study were to compare the inheritance of resistance to leaf scorch and root infection in a population that segregated for resistance to SCN and to identify the underlying quantitative trait loci (QTL). "Hartwig", a cultivar partially resistant to SDS leaf scorch, F. virguliforme root infection and SCN HG Type 1.3.6.7 was crossed with the partially susceptible cultivar "Flyer". Ninety-two F5-derived recombinant inbred lines and 144 markers were used for map development. Four QTL found in earlier studies were confirmed. One contributed resistance to leaf scorch on linkage group (LG) C2 (Satt277; P = 0.004, R 2 = 15%). Two on LG G underlay root infection at R8 (Satt038; P = 0.0001 R 2 = 28.1%; Satt115; P = 0.003, R 2 = 12.9%). The marker Satt038 was linked to rhg1 underlying resistance to SCN Hg Type 0. The fourth QTL was on LG D2 underlying resistance to root infection at R6 (Satt574; P = 0.001, R 2 = 10%). That QTL was in an interval previously associated with resistance to both SDS leaf scorch and SCN Hg Type 1.3.6.7. The QTL showed repulsion linkage with resistance to SCN that may explain the relative susceptibility to SDS of some SCN resistant cultivars. One additional QTL was discovered on LG G underlying resistance to SDS leaf scorch measured by disease index (Satt130; P = 0.003, R 2 = 13%). The loci and markers will provide tagged alleles with which to improve the breeding of cultivars combining resistances to SDS leaf scorch, root infection and SCN HG Type 1.3.6.7.

Original languageEnglish (US)
Pages (from-to)967-977
Number of pages11
JournalTheoretical and Applied Genetics
Volume116
Issue number7
DOIs
StatePublished - May 1 2008

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scorch
Heterodera glycines
Sudden Death
Soybeans
Cysts
Quantitative Trait Loci
loci
quantitative trait loci
Infection
infection
death
linkage groups
leaves
cultivars
Glycine
soybean sudden death syndrome
Fusarium solani
root rot
Fusarium
inbred lines

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Separate loci underlie resistance to root infection and leaf scorch during soybean sudden death syndrome. / Kazi, S.; Shultz, J.; Afzal, J.; Johnson, J.; Njiti, V. N.; Lightfoot, D. A.

In: Theoretical and Applied Genetics, Vol. 116, No. 7, 01.05.2008, p. 967-977.

Research output: Contribution to journalArticle

Kazi, S. ; Shultz, J. ; Afzal, J. ; Johnson, J. ; Njiti, V. N. ; Lightfoot, D. A. / Separate loci underlie resistance to root infection and leaf scorch during soybean sudden death syndrome. In: Theoretical and Applied Genetics. 2008 ; Vol. 116, No. 7. pp. 967-977.
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