Sensitivity enhancement of separated local field experiments: Application to membrane proteins

T. Gopinath, Raffaello Verardi, Nathaniel Traaseth, Gianluigi Veglia

Research output: Contribution to journalArticle

Abstract

Separated local field (SLF) experiments have been used for almost three decades to obtain structural information in solid-state NMR. These experiments resolve chemical shift anisotropy (CSA) from dipole-dipole interactions (dipolar couplings, DC) in isolated spin systems. Both CSA and DC data can be converted into orientational constraints to elucidate the secondary structure and topology of membrane proteins in oriented lipid bilayers. Here, we propose a new suite of sensitivity enhanced SLF pulse sequences to measure CSA and DC for aligned membrane proteins and liquid crystalline molecules that will decrease the time needed for data acquisition. We demonstrate the efficacy of these new sensitivity enhanced experiments using both a single crystal of N-acetyl leucine and a single pass membrane protein sarcolipin reconstituted in aligned lipid bicelles. These results lay the groundwork for the routine application of this methodology for studying the structure and topology of membrane proteins.

Original languageEnglish (US)
Pages (from-to)5089-5095
Number of pages7
JournalJournal of Physical Chemistry B
Volume114
Issue number15
DOIs
StatePublished - Apr 22 2010

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Membrane Proteins
Chemical shift
membranes
proteins
Proteins
Membranes
chemical equilibrium
Anisotropy
augmentation
sensitivity
anisotropy
lipids
topology
Experiments
Topology
dipoles
leucine
Lipid bilayers
Leucine
Lipids

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films

Cite this

Sensitivity enhancement of separated local field experiments : Application to membrane proteins. / Gopinath, T.; Verardi, Raffaello; Traaseth, Nathaniel; Veglia, Gianluigi.

In: Journal of Physical Chemistry B, Vol. 114, No. 15, 22.04.2010, p. 5089-5095.

Research output: Contribution to journalArticle

Gopinath, T. ; Verardi, Raffaello ; Traaseth, Nathaniel ; Veglia, Gianluigi. / Sensitivity enhancement of separated local field experiments : Application to membrane proteins. In: Journal of Physical Chemistry B. 2010 ; Vol. 114, No. 15. pp. 5089-5095.
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