Sensitivity enhanced heteronuclear correlation spectroscopy in multidimensional solid-state NMR of oriented systems via chemical shift coherences

T. Gopinath, Nathaniel Traaseth, Kaustubh Mote, Gianluigi Veglia

Research output: Contribution to journalArticle

Abstract

We present new sensitivity enhanced schemes for heteronuclear correlation spectroscopy (HETCOR) in solid-state NMR of oriented systems. These schemes will enhance the sensitivity of the HETCOR by 40% for the two-dimensional experiments (SE-HETCOR) and up to 180% for the 3D HETCOR-separated local field version (SE-PISEMAI-HETCOR). The signal enhancement is demonstrated for a single crystal of (15N)N-acetylleucine and the integral membrane protein sarcolipin oriented in lipid bicelles. These methods will significantly reduce the time needed to acquire multidimensional experiments for membrane proteins oriented in magnetically or mechanically aligned lipid bilayers as well as liquid crystalline materials.

Original languageEnglish (US)
Pages (from-to)5357-5363
Number of pages7
JournalJournal of the American Chemical Society
Volume132
Issue number15
DOIs
StatePublished - Apr 21 2010

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Chemical shift
Spectrum Analysis
Nuclear magnetic resonance
Spectroscopy
Membrane Proteins
Proteins
Membranes
Liquid Crystals
Lipid bilayers
Lipid Bilayers
Lipids
Experiments
Single crystals
Crystalline materials
Liquids

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Sensitivity enhanced heteronuclear correlation spectroscopy in multidimensional solid-state NMR of oriented systems via chemical shift coherences. / Gopinath, T.; Traaseth, Nathaniel; Mote, Kaustubh; Veglia, Gianluigi.

In: Journal of the American Chemical Society, Vol. 132, No. 15, 21.04.2010, p. 5357-5363.

Research output: Contribution to journalArticle

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