Self-association of hyaluronate segments in aqueous NaCl solution

Raymond E. Turner, Pinyen Lin, Mary Cowman

Research output: Contribution to journalArticle

Abstract

The potential for self-association by hyaluronate (HA) chains in 0.15 m NaCl was investigated, using low molecular weight HA segments as a model system. HA segments were derived from the polymer by controlled enzymatic digestion, and purified by gel filtration chromatography. Seven samples of narrow molecular weight distribution were analyzed by sensitivity-enhanced polyacrylamide gel electrophoresis, and found to have the following weight-average numbers of repeating disaccharide units: A, 90; B, 51; C, 38; D, 31; E, 23; F, 18; G, 13. The segment preparations were studied in 0.15 m NaCl by capillary viscometry, low angle laser light scattering, and circular dichroism spectroscopy. The data indicate concentration-dependent intermolecular association of short segments, and a capability for intramolecular association (hairpin formation) by larger HA segments.

Original languageEnglish (US)
Pages (from-to)484-495
Number of pages12
JournalArchives of Biochemistry and Biophysics
Volume265
Issue number2
DOIs
StatePublished - 1988

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Molecular Weight
Association reactions
Disaccharides
Circular Dichroism
Circular dichroism spectroscopy
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Digestion
Spectrum Analysis
Polymers
Lasers
Viscosity measurement
Molecular weight distribution
Chromatography
Electrophoresis
Light
Weights and Measures
Light scattering
Gels
Molecular weight

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Self-association of hyaluronate segments in aqueous NaCl solution. / Turner, Raymond E.; Lin, Pinyen; Cowman, Mary.

In: Archives of Biochemistry and Biophysics, Vol. 265, No. 2, 1988, p. 484-495.

Research output: Contribution to journalArticle

Turner, Raymond E. ; Lin, Pinyen ; Cowman, Mary. / Self-association of hyaluronate segments in aqueous NaCl solution. In: Archives of Biochemistry and Biophysics. 1988 ; Vol. 265, No. 2. pp. 484-495.
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