Self-assembly of polysaccharides gives rise to distinct mechanical signatures in marine gels

G. Pletikapić, H. Lannon, Ü Murvai, M. S Z Kellermayer, V. Svetličić, J. Brujic

    Research output: Contribution to journalArticle

    Abstract

    Marine-gel biopolymers were recently visualized at the molecular level using atomic force microscopy (AFM) to reveal fine fibril-forming networks with low to high degrees of cross-linking. In this work, we use force spectroscopy to quantify the intra- and intermolecular forces within the marine-gel network. Combining force measurements, AFM imaging, and the known chemical composition of marine gels allows us to identify the microscopic origins of distinct mechanical responses. At the single-fibril level, we uncover force-extension curves that resemble those of individual polysaccharide fibrils. They exhibit entropic elasticity followed by extensions associated with chair-to-boat transitions specific to the type of polysaccharide at high forces. Surprisingly, a low degree of cross-linking leads to sawtooth patterns that we attribute to the unraveling of polysaccharide entanglements. At a high degree of cross-linking, we observe force plateaus that arise from unzipping, as well as unwinding, of helical bundles. Finally, the complex 3D network structure gives rise to force staircases of increasing height that correspond to the hierarchical peeling of fibrils away from the junction zones. In addition, we show that these diverse mechanical responses also arise in reconstituted polysaccharide gels, which highlights their dominant role in the mechanical architecture of marine gels.

    Original languageEnglish (US)
    Pages (from-to)355-364
    Number of pages10
    JournalBiophysical Journal
    Volume107
    Issue number2
    DOIs
    StatePublished - Jul 15 2014

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    Polysaccharides
    Gels
    Atomic Force Microscopy
    Biopolymers
    Ships
    Elasticity
    Spectrum Analysis

    ASJC Scopus subject areas

    • Biophysics

    Cite this

    Pletikapić, G., Lannon, H., Murvai, Ü., Kellermayer, M. S. Z., Svetličić, V., & Brujic, J. (2014). Self-assembly of polysaccharides gives rise to distinct mechanical signatures in marine gels. Biophysical Journal, 107(2), 355-364. https://doi.org/10.1016/j.bpj.2014.04.065

    Self-assembly of polysaccharides gives rise to distinct mechanical signatures in marine gels. / Pletikapić, G.; Lannon, H.; Murvai, Ü; Kellermayer, M. S Z; Svetličić, V.; Brujic, J.

    In: Biophysical Journal, Vol. 107, No. 2, 15.07.2014, p. 355-364.

    Research output: Contribution to journalArticle

    Pletikapić, G, Lannon, H, Murvai, Ü, Kellermayer, MSZ, Svetličić, V & Brujic, J 2014, 'Self-assembly of polysaccharides gives rise to distinct mechanical signatures in marine gels', Biophysical Journal, vol. 107, no. 2, pp. 355-364. https://doi.org/10.1016/j.bpj.2014.04.065
    Pletikapić G, Lannon H, Murvai Ü, Kellermayer MSZ, Svetličić V, Brujic J. Self-assembly of polysaccharides gives rise to distinct mechanical signatures in marine gels. Biophysical Journal. 2014 Jul 15;107(2):355-364. https://doi.org/10.1016/j.bpj.2014.04.065
    Pletikapić, G. ; Lannon, H. ; Murvai, Ü ; Kellermayer, M. S Z ; Svetličić, V. ; Brujic, J. / Self-assembly of polysaccharides gives rise to distinct mechanical signatures in marine gels. In: Biophysical Journal. 2014 ; Vol. 107, No. 2. pp. 355-364.
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