Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component

Carina Hernandez, Jens J. Birktoft, Yoel P. Ohayon, Arun Richard Chandrasekaran, Hatem Abdallah, Ruojie Sha, Vivian Stojanoff, Chengde Mao, Nadrian Seeman

Research output: Contribution to journalArticle

Abstract

There is an increasing appreciation for structural diversity of DNA that is of interest to both DNA nanotechnology and basic biology. Here, we have explored how DNA responds to torsional stress by building on a previously reported two-turn DNA tensegrity triangle and demonstrating that we could introduce an extra nucleotide pair (np) into the original sequence without affecting assembly and crystallization. The extra np imposes a significant torsional stress, which is accommodated by global changes throughout the B-DNA duplex and the DNA lattice. The work reveals a near-atomic structure of naked DNA under a torsional stress of approximately 14%, and thus provides an example of DNA distortions that occur without a requirement for either an external energy source or the free energy available from protein or drug binding. DNA is stressed within the living cell, but it is difficult to observe its structural details. Here, Hernandez et al. incorporate stressed DNA within a self-assembled crystal lattice and determine its structure. The structure shows that no nucleotides are extruded, and the DNA remains as a B-form duplex.

Original languageEnglish (US)
JournalCell Chemical Biology
DOIs
StateAccepted/In press - 2017

Fingerprint

Self assembly
Crystals
DNA
Torsional stress
Nucleotides
B-Form DNA
Nanotechnology
Crystallization
Crystal lattices
Free energy
Cells
Pharmaceutical Preparations

Keywords

  • Designed crystals
  • Self-assembled 3D DNA crystals
  • Structural DNA nanotechnology
  • Torsionally stressed DNA
  • X-ray diffraction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Hernandez, C., Birktoft, J. J., Ohayon, Y. P., Chandrasekaran, A. R., Abdallah, H., Sha, R., ... Seeman, N. (Accepted/In press). Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component. Cell Chemical Biology. https://doi.org/10.1016/j.chembiol.2017.08.018

Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component. / Hernandez, Carina; Birktoft, Jens J.; Ohayon, Yoel P.; Chandrasekaran, Arun Richard; Abdallah, Hatem; Sha, Ruojie; Stojanoff, Vivian; Mao, Chengde; Seeman, Nadrian.

In: Cell Chemical Biology, 2017.

Research output: Contribution to journalArticle

Hernandez, C, Birktoft, JJ, Ohayon, YP, Chandrasekaran, AR, Abdallah, H, Sha, R, Stojanoff, V, Mao, C & Seeman, N 2017, 'Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component', Cell Chemical Biology. https://doi.org/10.1016/j.chembiol.2017.08.018
Hernandez, Carina ; Birktoft, Jens J. ; Ohayon, Yoel P. ; Chandrasekaran, Arun Richard ; Abdallah, Hatem ; Sha, Ruojie ; Stojanoff, Vivian ; Mao, Chengde ; Seeman, Nadrian. / Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component. In: Cell Chemical Biology. 2017.
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