Selective effect of inhibitors on inner mitochondrial membrane channels

Yuri N. Antonenko, Kathleen W. Kinnally, Sean Perini, Henry Tedeschi

Research output: Contribution to journalArticle

Abstract

The effect of amphiphilic cationic drugs on the channel activity of the mitochondrial inner membrane was examined with patch-clamp techniques. The therapeutic drugs amiodarone, propranolol and quinine reduced the probability of being open for the multiconductance channel (MCC) activity (levels from 30 pS to over I nS). While amiodarone decreased the probability of being open for the voltage dependent ∼ 100 pS channel, it increased the conductance 42 ± 20% (mean ± SD, n = 6) with no significant change in mean open time. Similar results were obtained with propranolol. These data indicate that the ∼ 100 pS channel is distinct from MCC activity.

Original languageEnglish (US)
Pages (from-to)89-93
Number of pages5
JournalFEBS Letters
Volume285
Issue number1
DOIs
StatePublished - Jul 8 1991

Fingerprint

Amiodarone
Mitochondrial Membranes
Ion Channels
Propranolol
Quinine
Clamping devices
Patch-Clamp Techniques
Pharmaceutical Preparations
Membranes
Electric potential
Therapeutics

Keywords

  • Inner mitochondrial membrane
  • Mitochondrial channel
  • Patch-clamp
  • Pharmacology

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Antonenko, Y. N., Kinnally, K. W., Perini, S., & Tedeschi, H. (1991). Selective effect of inhibitors on inner mitochondrial membrane channels. FEBS Letters, 285(1), 89-93. https://doi.org/10.1016/0014-5793(91)80731-H

Selective effect of inhibitors on inner mitochondrial membrane channels. / Antonenko, Yuri N.; Kinnally, Kathleen W.; Perini, Sean; Tedeschi, Henry.

In: FEBS Letters, Vol. 285, No. 1, 08.07.1991, p. 89-93.

Research output: Contribution to journalArticle

Antonenko, YN, Kinnally, KW, Perini, S & Tedeschi, H 1991, 'Selective effect of inhibitors on inner mitochondrial membrane channels', FEBS Letters, vol. 285, no. 1, pp. 89-93. https://doi.org/10.1016/0014-5793(91)80731-H
Antonenko, Yuri N. ; Kinnally, Kathleen W. ; Perini, Sean ; Tedeschi, Henry. / Selective effect of inhibitors on inner mitochondrial membrane channels. In: FEBS Letters. 1991 ; Vol. 285, No. 1. pp. 89-93.
@article{636e6fcffe214c6f971c42597dfa9825,
title = "Selective effect of inhibitors on inner mitochondrial membrane channels",
abstract = "The effect of amphiphilic cationic drugs on the channel activity of the mitochondrial inner membrane was examined with patch-clamp techniques. The therapeutic drugs amiodarone, propranolol and quinine reduced the probability of being open for the multiconductance channel (MCC) activity (levels from 30 pS to over I nS). While amiodarone decreased the probability of being open for the voltage dependent ∼ 100 pS channel, it increased the conductance 42 ± 20{\%} (mean ± SD, n = 6) with no significant change in mean open time. Similar results were obtained with propranolol. These data indicate that the ∼ 100 pS channel is distinct from MCC activity.",
keywords = "Inner mitochondrial membrane, Mitochondrial channel, Patch-clamp, Pharmacology",
author = "Antonenko, {Yuri N.} and Kinnally, {Kathleen W.} and Sean Perini and Henry Tedeschi",
year = "1991",
month = "7",
day = "8",
doi = "10.1016/0014-5793(91)80731-H",
language = "English (US)",
volume = "285",
pages = "89--93",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Selective effect of inhibitors on inner mitochondrial membrane channels

AU - Antonenko, Yuri N.

AU - Kinnally, Kathleen W.

AU - Perini, Sean

AU - Tedeschi, Henry

PY - 1991/7/8

Y1 - 1991/7/8

N2 - The effect of amphiphilic cationic drugs on the channel activity of the mitochondrial inner membrane was examined with patch-clamp techniques. The therapeutic drugs amiodarone, propranolol and quinine reduced the probability of being open for the multiconductance channel (MCC) activity (levels from 30 pS to over I nS). While amiodarone decreased the probability of being open for the voltage dependent ∼ 100 pS channel, it increased the conductance 42 ± 20% (mean ± SD, n = 6) with no significant change in mean open time. Similar results were obtained with propranolol. These data indicate that the ∼ 100 pS channel is distinct from MCC activity.

AB - The effect of amphiphilic cationic drugs on the channel activity of the mitochondrial inner membrane was examined with patch-clamp techniques. The therapeutic drugs amiodarone, propranolol and quinine reduced the probability of being open for the multiconductance channel (MCC) activity (levels from 30 pS to over I nS). While amiodarone decreased the probability of being open for the voltage dependent ∼ 100 pS channel, it increased the conductance 42 ± 20% (mean ± SD, n = 6) with no significant change in mean open time. Similar results were obtained with propranolol. These data indicate that the ∼ 100 pS channel is distinct from MCC activity.

KW - Inner mitochondrial membrane

KW - Mitochondrial channel

KW - Patch-clamp

KW - Pharmacology

UR - http://www.scopus.com/inward/record.url?scp=0025856182&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025856182&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(91)80731-H

DO - 10.1016/0014-5793(91)80731-H

M3 - Article

C2 - 1712321

AN - SCOPUS:0025856182

VL - 285

SP - 89

EP - 93

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -