Selective, controllable, and reversible aggregation of polystyrene latex microspheres via DNA hybridization

Phillip H. Rogers, Eric Michel, Carl A. Bauer, Stephen Vanderet, Daniel Hansen, Bradley K. Roberts, Antoine Calvez, Jackson B. Crews, Kwok O. Lau, Alistair Wood, David J. Pine, Peter V. Schwartz

Research output: Contribution to journalArticle

Abstract

The directed three-dimensional self-assembly of microstructures and nanostructures through the selective hybridization of DNA is the focus of great interest toward the fabrication of new materials. Single-stranded DNA is covalently attached to polystyrene latex microspheres. Single-stranded DNA can function as a sequence-selective Velcro by only bonding to another strand of DNA that has a complementary sequence. The attachment of the DNA increases the charge stabilization of the microspheres and allows controllable aggregation of microspheres by hybridization of complementary DNA sequences. In a mixture of microspheres derivatized with different sequences of DNA, microspheres with complementary DNA form aggregates, while microspheres with noncomplementary sequences remain suspended. The process is reversible by heating, with a characteristic "aggregate dissociation temperature" that is predictably dependent on salt concentration, and the evolution of aggregate dissociation with temperature is observed with optical microscopy.

Original languageEnglish (US)
Pages (from-to)5562-5569
Number of pages8
JournalLangmuir
Volume21
Issue number12
DOIs
StatePublished - Jun 7 2005

Fingerprint

latex
Latexes
Microspheres
Polystyrenes
polystyrene
DNA
Agglomeration
deoxyribonucleic acid
complementary DNA
Single-Stranded DNA
Complementary DNA
dissociation
DNA sequences
strands
Self assembly
Optical microscopy
attachment
styrofoam
self assembly
Nanostructures

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Rogers, P. H., Michel, E., Bauer, C. A., Vanderet, S., Hansen, D., Roberts, B. K., ... Schwartz, P. V. (2005). Selective, controllable, and reversible aggregation of polystyrene latex microspheres via DNA hybridization. Langmuir, 21(12), 5562-5569. https://doi.org/10.1021/la046790y

Selective, controllable, and reversible aggregation of polystyrene latex microspheres via DNA hybridization. / Rogers, Phillip H.; Michel, Eric; Bauer, Carl A.; Vanderet, Stephen; Hansen, Daniel; Roberts, Bradley K.; Calvez, Antoine; Crews, Jackson B.; Lau, Kwok O.; Wood, Alistair; Pine, David J.; Schwartz, Peter V.

In: Langmuir, Vol. 21, No. 12, 07.06.2005, p. 5562-5569.

Research output: Contribution to journalArticle

Rogers, PH, Michel, E, Bauer, CA, Vanderet, S, Hansen, D, Roberts, BK, Calvez, A, Crews, JB, Lau, KO, Wood, A, Pine, DJ & Schwartz, PV 2005, 'Selective, controllable, and reversible aggregation of polystyrene latex microspheres via DNA hybridization', Langmuir, vol. 21, no. 12, pp. 5562-5569. https://doi.org/10.1021/la046790y
Rogers PH, Michel E, Bauer CA, Vanderet S, Hansen D, Roberts BK et al. Selective, controllable, and reversible aggregation of polystyrene latex microspheres via DNA hybridization. Langmuir. 2005 Jun 7;21(12):5562-5569. https://doi.org/10.1021/la046790y
Rogers, Phillip H. ; Michel, Eric ; Bauer, Carl A. ; Vanderet, Stephen ; Hansen, Daniel ; Roberts, Bradley K. ; Calvez, Antoine ; Crews, Jackson B. ; Lau, Kwok O. ; Wood, Alistair ; Pine, David J. ; Schwartz, Peter V. / Selective, controllable, and reversible aggregation of polystyrene latex microspheres via DNA hybridization. In: Langmuir. 2005 ; Vol. 21, No. 12. pp. 5562-5569.
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