Saturation of the biological response to orthodontic forces and its effect on the rate of tooth movement

M. Alikhani, B. Alyami, I. S. Lee, S. Almoammar, T. Vongthongleur, M. Alikhani, S. Alansari, C. Sangsuwon, M. Y. Chou, E. Khoo, A. Boskey, Cristina Teixeira

Research output: Contribution to journalArticle

Abstract

Objectives: Investigate the expression and activity of inflammatory markers in response to different magnitudes of orthodontic forces and correlate this response with other molecular and cellular events during orthodontic tooth movement. Setting and Sample Population: CTOR Laboratory; 245 Sprague Dawley male rats. Methods and Materials: Control, sham, and 5 different experimental groups received different magnitudes of force on the right maxillary first molar using a coil spring. In the sham group, the spring was not activated. Control group did not receive any appliance. At days 1, 3, 7, 14, and 28, the maxillae were collected for RNA and protein analysis, immunohistochemistry, and micro-CT. Results: There was a linear relation between the force and the level of cytokine expression at lower magnitudes of force. Higher magnitudes of force did not increase the expression of cytokines. Activity of CCL2, CCL5, IL-1, TNF-α, RANKL, and number of osteoclasts reached a saturation point in response to higher magnitudes of force, with unchanged rate of tooth movement. Conclusion: After a certain magnitude of force, there is a saturation in the biological response, and higher forces do not increase inflammatory markers, osteoclasts, nor the amount of tooth movement. Therefore, higher forces to accelerate the rate of tooth movement are not justified.

Original languageEnglish (US)
Pages (from-to)8-17
Number of pages10
JournalOrthodontics and Craniofacial Research
Volume18
Issue numberS1
DOIs
StatePublished - Apr 1 2015

Fingerprint

Tooth Movement Techniques
Orthodontics
Osteoclasts
Cytokines
Maxilla
Interleukin-1
Sprague Dawley Rats
Immunohistochemistry
RNA
Control Groups
Population
Proteins

Keywords

  • Cytokines
  • Force
  • Gene expression
  • Orthodontics
  • Osteoclasts
  • Tooth movement

ASJC Scopus subject areas

  • Orthodontics
  • Oral Surgery
  • Otorhinolaryngology
  • Surgery

Cite this

Saturation of the biological response to orthodontic forces and its effect on the rate of tooth movement. / Alikhani, M.; Alyami, B.; Lee, I. S.; Almoammar, S.; Vongthongleur, T.; Alikhani, M.; Alansari, S.; Sangsuwon, C.; Chou, M. Y.; Khoo, E.; Boskey, A.; Teixeira, Cristina.

In: Orthodontics and Craniofacial Research, Vol. 18, No. S1, 01.04.2015, p. 8-17.

Research output: Contribution to journalArticle

Alikhani, M, Alyami, B, Lee, IS, Almoammar, S, Vongthongleur, T, Alikhani, M, Alansari, S, Sangsuwon, C, Chou, MY, Khoo, E, Boskey, A & Teixeira, C 2015, 'Saturation of the biological response to orthodontic forces and its effect on the rate of tooth movement', Orthodontics and Craniofacial Research, vol. 18, no. S1, pp. 8-17. https://doi.org/10.1111/ocr.12090
Alikhani, M. ; Alyami, B. ; Lee, I. S. ; Almoammar, S. ; Vongthongleur, T. ; Alikhani, M. ; Alansari, S. ; Sangsuwon, C. ; Chou, M. Y. ; Khoo, E. ; Boskey, A. ; Teixeira, Cristina. / Saturation of the biological response to orthodontic forces and its effect on the rate of tooth movement. In: Orthodontics and Craniofacial Research. 2015 ; Vol. 18, No. S1. pp. 8-17.
@article{28b06a09afe84d93aaf79180d7f06ce9,
title = "Saturation of the biological response to orthodontic forces and its effect on the rate of tooth movement",
abstract = "Objectives: Investigate the expression and activity of inflammatory markers in response to different magnitudes of orthodontic forces and correlate this response with other molecular and cellular events during orthodontic tooth movement. Setting and Sample Population: CTOR Laboratory; 245 Sprague Dawley male rats. Methods and Materials: Control, sham, and 5 different experimental groups received different magnitudes of force on the right maxillary first molar using a coil spring. In the sham group, the spring was not activated. Control group did not receive any appliance. At days 1, 3, 7, 14, and 28, the maxillae were collected for RNA and protein analysis, immunohistochemistry, and micro-CT. Results: There was a linear relation between the force and the level of cytokine expression at lower magnitudes of force. Higher magnitudes of force did not increase the expression of cytokines. Activity of CCL2, CCL5, IL-1, TNF-α, RANKL, and number of osteoclasts reached a saturation point in response to higher magnitudes of force, with unchanged rate of tooth movement. Conclusion: After a certain magnitude of force, there is a saturation in the biological response, and higher forces do not increase inflammatory markers, osteoclasts, nor the amount of tooth movement. Therefore, higher forces to accelerate the rate of tooth movement are not justified.",
keywords = "Cytokines, Force, Gene expression, Orthodontics, Osteoclasts, Tooth movement",
author = "M. Alikhani and B. Alyami and Lee, {I. S.} and S. Almoammar and T. Vongthongleur and M. Alikhani and S. Alansari and C. Sangsuwon and Chou, {M. Y.} and E. Khoo and A. Boskey and Cristina Teixeira",
year = "2015",
month = "4",
day = "1",
doi = "10.1111/ocr.12090",
language = "English (US)",
volume = "18",
pages = "8--17",
journal = "Orthodontics and Craniofacial Research",
issn = "1601-6335",
publisher = "Wiley-Blackwell",
number = "S1",

}

TY - JOUR

T1 - Saturation of the biological response to orthodontic forces and its effect on the rate of tooth movement

AU - Alikhani, M.

AU - Alyami, B.

AU - Lee, I. S.

AU - Almoammar, S.

AU - Vongthongleur, T.

AU - Alikhani, M.

AU - Alansari, S.

AU - Sangsuwon, C.

AU - Chou, M. Y.

AU - Khoo, E.

AU - Boskey, A.

AU - Teixeira, Cristina

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Objectives: Investigate the expression and activity of inflammatory markers in response to different magnitudes of orthodontic forces and correlate this response with other molecular and cellular events during orthodontic tooth movement. Setting and Sample Population: CTOR Laboratory; 245 Sprague Dawley male rats. Methods and Materials: Control, sham, and 5 different experimental groups received different magnitudes of force on the right maxillary first molar using a coil spring. In the sham group, the spring was not activated. Control group did not receive any appliance. At days 1, 3, 7, 14, and 28, the maxillae were collected for RNA and protein analysis, immunohistochemistry, and micro-CT. Results: There was a linear relation between the force and the level of cytokine expression at lower magnitudes of force. Higher magnitudes of force did not increase the expression of cytokines. Activity of CCL2, CCL5, IL-1, TNF-α, RANKL, and number of osteoclasts reached a saturation point in response to higher magnitudes of force, with unchanged rate of tooth movement. Conclusion: After a certain magnitude of force, there is a saturation in the biological response, and higher forces do not increase inflammatory markers, osteoclasts, nor the amount of tooth movement. Therefore, higher forces to accelerate the rate of tooth movement are not justified.

AB - Objectives: Investigate the expression and activity of inflammatory markers in response to different magnitudes of orthodontic forces and correlate this response with other molecular and cellular events during orthodontic tooth movement. Setting and Sample Population: CTOR Laboratory; 245 Sprague Dawley male rats. Methods and Materials: Control, sham, and 5 different experimental groups received different magnitudes of force on the right maxillary first molar using a coil spring. In the sham group, the spring was not activated. Control group did not receive any appliance. At days 1, 3, 7, 14, and 28, the maxillae were collected for RNA and protein analysis, immunohistochemistry, and micro-CT. Results: There was a linear relation between the force and the level of cytokine expression at lower magnitudes of force. Higher magnitudes of force did not increase the expression of cytokines. Activity of CCL2, CCL5, IL-1, TNF-α, RANKL, and number of osteoclasts reached a saturation point in response to higher magnitudes of force, with unchanged rate of tooth movement. Conclusion: After a certain magnitude of force, there is a saturation in the biological response, and higher forces do not increase inflammatory markers, osteoclasts, nor the amount of tooth movement. Therefore, higher forces to accelerate the rate of tooth movement are not justified.

KW - Cytokines

KW - Force

KW - Gene expression

KW - Orthodontics

KW - Osteoclasts

KW - Tooth movement

UR - http://www.scopus.com/inward/record.url?scp=84926480511&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84926480511&partnerID=8YFLogxK

U2 - 10.1111/ocr.12090

DO - 10.1111/ocr.12090

M3 - Article

C2 - 25865529

AN - SCOPUS:84926480511

VL - 18

SP - 8

EP - 17

JO - Orthodontics and Craniofacial Research

JF - Orthodontics and Craniofacial Research

SN - 1601-6335

IS - S1

ER -