Salivary non-immunoglobulin agglutinin inhibits human leukocyte elastase digestion of acidic proline-rich salivary proteins

R. J. Boackle, S. L. Dutton, H. Fei, J. Vesely, Daniel Malamud, A. R. Furness

Research output: Contribution to journalArticle

Abstract

Saliva contains acidic proline-rich salivary proteins that are involved in the formation of the salivary pellicle coating supragingival tooth surfaces. However, human leukocyte elastase, arriving in gingival exudates from inflamed periodontal tissues, degrades the acidic proline-rich salivary proteins, preventing binding to hydroxylapatite surfaces. Here it is reported that high-molecular-weight non-immunoglobulin salivary agglutinin inhibited the proteolytic action of human leukocyte elastase on purified acidic proline-rich salivary proteins. Inhibition was eliminated with monoclonal antibody to a protein determinant on the salivary agglutinin. The addition of antibody against salivary agglutinin blocked the inhibitory effect of parotid saliva on exogenously applied human leukocyte elastase, allowing for the elastase-mediated digestion of the salivary acidic proline-rich salivary proteins. Salivary agglutinin, therefore, is a physiologically important inhibitor of human leukocyte elastase and is able to inhibit elastase-mediated digestion of salivary acidic proline-rich proteins.

Original languageEnglish (US)
Pages (from-to)1550-1554
Number of pages5
JournalJournal of Dental Research
Volume80
Issue number6
StatePublished - 2001

Fingerprint

Salivary Proline-Rich Proteins
Leukocyte Elastase
Agglutinins
Digestion
Pancreatic Elastase
Saliva
Dental Pellicle
Gingival Crevicular Fluid
Durapatite
Proline
Protein Binding
Tooth
Molecular Weight
Monoclonal Antibodies
Antibodies
Proteins

Keywords

  • Acidic salivary proline-rich proteins
  • High molecular weight non-immunoglobulin salivary agglutinin (salivary agglutinin)
  • Human immunodeficiency virus
  • Human leukocyte elastase
  • Periodontal disease
  • Salivary leukocyte protease inhibitor

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Salivary non-immunoglobulin agglutinin inhibits human leukocyte elastase digestion of acidic proline-rich salivary proteins. / Boackle, R. J.; Dutton, S. L.; Fei, H.; Vesely, J.; Malamud, Daniel; Furness, A. R.

In: Journal of Dental Research, Vol. 80, No. 6, 2001, p. 1550-1554.

Research output: Contribution to journalArticle

Boackle, R. J. ; Dutton, S. L. ; Fei, H. ; Vesely, J. ; Malamud, Daniel ; Furness, A. R. / Salivary non-immunoglobulin agglutinin inhibits human leukocyte elastase digestion of acidic proline-rich salivary proteins. In: Journal of Dental Research. 2001 ; Vol. 80, No. 6. pp. 1550-1554.
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AU - Vesely, J.

AU - Malamud, Daniel

AU - Furness, A. R.

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