Salivary gland cytokine expression in NOD and normal BALB/c mice

Seiichi Yamano, Jane C. Atkinson, Bruce J. Baum, Philip C. Fox

Research output: Contribution to journalArticle

Abstract

The autoimmune diabetes-prone nonobese diabetic (NOD) mouse develops a chronic lymphocytic infiltration of endocrine and exocrine glands. The objectives of this study were to characterize the salivary immune infiltration and cytokine expression of NOD mice and compare these findings to those of normal BALB/c mice. A decline in salivary flow rates in NOD mice began between 8 and 12 weeks of age. At this same time lymphocytic foci are detectable in the salivary glands. Lymphocytic infiltration in the salivary glands of NOD mice increased with age and simultaneously salivary function declined. No lymphocytic infiltration was seen in BALB/c salivary tissues. Messenger RNA expression of several inflammatory cytokines, including interleukin-1β (IL-1β), IL-2, IL-10, interferon-γ, and tumor necrosis factor-α was detected in the submandibular glands of both NOD and BALB/c mice by the reverse transcription polymerase chain reaction. IL-4 synthesis was also present in some tissues. Immunohistochemical analysis demonstrated the intense expression of inflammatory cytokines within lymphocytic infiltrates and epithelial cells of all NOD mice. Minimal expression of the same cytokines was detected only occasionally in BALB/c tissues stained in parallel. These results demonstrate cytokine expression in the salivary glands of normal mice and suggest that the overexpression of these inflammatory cytokines is likely involved in the development and progression of the organ-localized autoimmunity in the salivary glands of NOD mice.

Original languageEnglish (US)
Pages (from-to)265-275
Number of pages11
JournalClinical Immunology
Volume92
Issue number3
DOIs
StatePublished - Sep 1999

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Inbred NOD Mouse
Salivary Glands
Cytokines
Exocrine Glands
Endocrine Glands
Submandibular Gland
Autoimmunity
Type 1 Diabetes Mellitus
Interleukin-1
Interleukin-4
Interleukin-10
Interferons
Reverse Transcription
Interleukin-2
Tumor Necrosis Factor-alpha
Epithelial Cells
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Autoimmunity
  • Cytokine
  • NOD mouse
  • Salivary gland
  • Sjogren's syndrome

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Salivary gland cytokine expression in NOD and normal BALB/c mice. / Yamano, Seiichi; Atkinson, Jane C.; Baum, Bruce J.; Fox, Philip C.

In: Clinical Immunology, Vol. 92, No. 3, 09.1999, p. 265-275.

Research output: Contribution to journalArticle

Yamano, Seiichi ; Atkinson, Jane C. ; Baum, Bruce J. ; Fox, Philip C. / Salivary gland cytokine expression in NOD and normal BALB/c mice. In: Clinical Immunology. 1999 ; Vol. 92, No. 3. pp. 265-275.
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