SAF-A/hnRNP-U localization in interphase and metaphase

A. S. Kukalev, I. B. Lobov, P. Percipalle, O. I. Podgornaya

Research output: Contribution to journalArticle

Abstract

SAF-A/hnRNP U is an abundant nuclear protein that interacts specifically with nuclear matrix attachment region DNA (MAR) and RNA as a component of hnRNPs. SAF-A/hnRNP U was also shown to specifically bind mouse major satellite DNA (satMa). Antibodies against SAF-A and GFP-fusion constructs were used in the current work in order to trace SAF-A localization. In accordance with its diverse nucleic acid binding specificity, SAF-A was found to be localized in three different domains: outside the chromosomes, on the surface of the chromosome arms (probably MARs), and in the centromere region where it apparently binds specifically to the satMa. GFP-fusion constructs with different SAF-A/hnRNP U domains confirms the functional significance of the protein's functional domains in interphase cells. In telophase cells, the anti-SAF-A antibody signal appeared as a kind of network covering unfolded chromosomes.

Original languageEnglish (US)
Pages (from-to)288-297
Number of pages10
JournalCytogenetic and Genome Research
Volume124
Issue number3-4
DOIs
StatePublished - Jun 1 2009

Keywords

  • Chromosome territory formation
  • Localization
  • MAR binding
  • Nuclear matrix
  • SAF-A/hnRNP-U
  • Satellite DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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