Root Regeneration Triggers an Embryo-like Sequence Guided by Hormonal Interactions

Idan Efroni, Alison Mello, Tal Nawy, Pui Leng Ip, Ramin Rahni, Nicholas Delrose, Ashley Powers, Rahul Satija, Kenneth Birnbaum

Research output: Contribution to journalArticle

Abstract

Plant roots can regenerate after excision of their tip, including the stem cell niche. To determine which developmental program mediates such repair, we applied a combination of lineage tracing, single-cell RNA sequencing, and marker analysis to test different models of tissue reassembly. We show that multiple cell types can reconstitute stem cells, demonstrating the latent potential of untreated plant cells. The transcriptome of regenerating cells prior to stem cell activation resembles that of an embryonic root progenitor. Regeneration defects are more severe in embryonic than in adult root mutants. Furthermore, the signaling domains of the hormones auxin and cytokinin mirror their embryonic dynamics and manipulation of both hormones alters the position of new tissues and stem cell niche markers. Our findings suggest that plant root regeneration follows, on a larger scale, the developmental stages of embryonic patterning and is guided by spatial information provided by complementary hormone domains.

Original languageEnglish (US)
Pages (from-to)1721-1733
Number of pages13
JournalCell
Volume165
Issue number7
DOIs
StatePublished - Jun 16 2016

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Stem cells
Stem Cell Niche
Regeneration
Plant Roots
Embryonic Structures
Hormones
Stem Cells
RNA Sequence Analysis
Cytokinins
Indoleacetic Acids
Plant Cells
Tissue
Transcriptome
Mirrors
Repair
Chemical activation
RNA
Defects

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Efroni, I., Mello, A., Nawy, T., Ip, P. L., Rahni, R., Delrose, N., ... Birnbaum, K. (2016). Root Regeneration Triggers an Embryo-like Sequence Guided by Hormonal Interactions. Cell, 165(7), 1721-1733. https://doi.org/10.1016/j.cell.2016.04.046

Root Regeneration Triggers an Embryo-like Sequence Guided by Hormonal Interactions. / Efroni, Idan; Mello, Alison; Nawy, Tal; Ip, Pui Leng; Rahni, Ramin; Delrose, Nicholas; Powers, Ashley; Satija, Rahul; Birnbaum, Kenneth.

In: Cell, Vol. 165, No. 7, 16.06.2016, p. 1721-1733.

Research output: Contribution to journalArticle

Efroni, I, Mello, A, Nawy, T, Ip, PL, Rahni, R, Delrose, N, Powers, A, Satija, R & Birnbaum, K 2016, 'Root Regeneration Triggers an Embryo-like Sequence Guided by Hormonal Interactions', Cell, vol. 165, no. 7, pp. 1721-1733. https://doi.org/10.1016/j.cell.2016.04.046
Efroni I, Mello A, Nawy T, Ip PL, Rahni R, Delrose N et al. Root Regeneration Triggers an Embryo-like Sequence Guided by Hormonal Interactions. Cell. 2016 Jun 16;165(7):1721-1733. https://doi.org/10.1016/j.cell.2016.04.046
Efroni, Idan ; Mello, Alison ; Nawy, Tal ; Ip, Pui Leng ; Rahni, Ramin ; Delrose, Nicholas ; Powers, Ashley ; Satija, Rahul ; Birnbaum, Kenneth. / Root Regeneration Triggers an Embryo-like Sequence Guided by Hormonal Interactions. In: Cell. 2016 ; Vol. 165, No. 7. pp. 1721-1733.
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