Role of VEGF in small bowel adaptation after resection: The adaptive response is angiogenesis dependent

Jignesh K. Parvadia, Sundeep G. Keswani, Sachin Vaikunth, Arturo R. Maldonado, A. Marwan, Wolfgang Stehr, Christopher Erwin, Eva Uzvolgyi, Brad W. Warner, Seiichi Yamano, Norton Taichman, Timothy M. Crombleholme

Research output: Contribution to journalArticle

Abstract

Previous work in our group has demonstrated that mouse salivary gland has the highest concentration of salivary-derived VEGF protein compared with other organs and is essential for normal palatal mucosal wound healing. We hypothesize that salivary VEGF plays an important role in maintaining the integrity of the gastrointestinal mucosa following small bowel resection (SBR). Thirty-five 8- to 10-wk-old C57BL/6 female mice were divided into seven treatment groups: 1) sham (transaction and anastomosis, n = 5); 2) SBR (n = 8); 3) sialoadenectomy and small bowel resection (SAL+SBR, n = 8); 4) sialoadenectomy and small bowel resection with EGF supplementation (SAL+SBR+EGF, n = 9); 5) sialoadenectomy and small bowel resection with VEGF supplementation (SAL+SBR+VEGF, n = 9); 6) sialoadenectomy and small bowel resection supplemented with EGF and VEGF (SAL+SBR+VEGF+EGF, n = 6); 7) selective inhibition of VEGF in the submandibular gland by Ad-VEGF-Trap following small bowel resection (Ad-VEGF-Trap+SBR, n = 7). Adaptation was after 3 days by ileal villus height and crypt depth. The microvascular response was evaluated by CD31 immunostaining and for villus-vessel area ratio by FITC-labeled von Willebrand factor immunostaining. The adaptive response after SBR was significantly attenuated in the SAL group in terms of villus height (250.4 ± 8.816 vs. 310 ± 19.35, P = 0.01) and crypt depth (100.021 ± 4.025 vs. 120.541 ± 2.82, P = 0.01). This response was partially corrected by orogastric VEGF or EGF alone. The adaptive response was completely restored when both were administered together, suggesting that salivary VEGF and EGF both contribute to intestinal adaptation. VEGF increases the vascular density (6.4 ± 0.29 vs. 6.1 ± 0.29 vs. 5.96 ± 0.20) and villus-vessel area ratio (0.713 ± 0.01 vs. 0.73 ± 0.01) in the adapting bowel. Supplementation of both EGF and VEGF fully rescues adaptation, suggesting that the adaptive response may be dependent on VEGF-driven angiogenesis. These results support a previously unrecognized role for VEGF in the small bowel adaptive response.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume293
Issue number3
DOIs
StatePublished - Sep 2007

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Vascular Endothelial Growth Factor A
Epidermal Growth Factor
Fluorescein-5-isothiocyanate
Submandibular Gland
von Willebrand Factor
Salivary Glands
Wound Healing
Blood Vessels
Mucous Membrane

Keywords

  • EGF
  • Sialoadenectomy
  • Small bowel resection
  • VEGF

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Role of VEGF in small bowel adaptation after resection : The adaptive response is angiogenesis dependent. / Parvadia, Jignesh K.; Keswani, Sundeep G.; Vaikunth, Sachin; Maldonado, Arturo R.; Marwan, A.; Stehr, Wolfgang; Erwin, Christopher; Uzvolgyi, Eva; Warner, Brad W.; Yamano, Seiichi; Taichman, Norton; Crombleholme, Timothy M.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 293, No. 3, 09.2007.

Research output: Contribution to journalArticle

Parvadia, JK, Keswani, SG, Vaikunth, S, Maldonado, AR, Marwan, A, Stehr, W, Erwin, C, Uzvolgyi, E, Warner, BW, Yamano, S, Taichman, N & Crombleholme, TM 2007, 'Role of VEGF in small bowel adaptation after resection: The adaptive response is angiogenesis dependent', American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 293, no. 3. https://doi.org/10.1152/ajpgi.00572.2006
Parvadia, Jignesh K. ; Keswani, Sundeep G. ; Vaikunth, Sachin ; Maldonado, Arturo R. ; Marwan, A. ; Stehr, Wolfgang ; Erwin, Christopher ; Uzvolgyi, Eva ; Warner, Brad W. ; Yamano, Seiichi ; Taichman, Norton ; Crombleholme, Timothy M. / Role of VEGF in small bowel adaptation after resection : The adaptive response is angiogenesis dependent. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2007 ; Vol. 293, No. 3.
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AU - Keswani, Sundeep G.

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AU - Maldonado, Arturo R.

AU - Marwan, A.

AU - Stehr, Wolfgang

AU - Erwin, Christopher

AU - Uzvolgyi, Eva

AU - Warner, Brad W.

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AU - Crombleholme, Timothy M.

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N2 - Previous work in our group has demonstrated that mouse salivary gland has the highest concentration of salivary-derived VEGF protein compared with other organs and is essential for normal palatal mucosal wound healing. We hypothesize that salivary VEGF plays an important role in maintaining the integrity of the gastrointestinal mucosa following small bowel resection (SBR). Thirty-five 8- to 10-wk-old C57BL/6 female mice were divided into seven treatment groups: 1) sham (transaction and anastomosis, n = 5); 2) SBR (n = 8); 3) sialoadenectomy and small bowel resection (SAL+SBR, n = 8); 4) sialoadenectomy and small bowel resection with EGF supplementation (SAL+SBR+EGF, n = 9); 5) sialoadenectomy and small bowel resection with VEGF supplementation (SAL+SBR+VEGF, n = 9); 6) sialoadenectomy and small bowel resection supplemented with EGF and VEGF (SAL+SBR+VEGF+EGF, n = 6); 7) selective inhibition of VEGF in the submandibular gland by Ad-VEGF-Trap following small bowel resection (Ad-VEGF-Trap+SBR, n = 7). Adaptation was after 3 days by ileal villus height and crypt depth. The microvascular response was evaluated by CD31 immunostaining and for villus-vessel area ratio by FITC-labeled von Willebrand factor immunostaining. The adaptive response after SBR was significantly attenuated in the SAL group in terms of villus height (250.4 ± 8.816 vs. 310 ± 19.35, P = 0.01) and crypt depth (100.021 ± 4.025 vs. 120.541 ± 2.82, P = 0.01). This response was partially corrected by orogastric VEGF or EGF alone. The adaptive response was completely restored when both were administered together, suggesting that salivary VEGF and EGF both contribute to intestinal adaptation. VEGF increases the vascular density (6.4 ± 0.29 vs. 6.1 ± 0.29 vs. 5.96 ± 0.20) and villus-vessel area ratio (0.713 ± 0.01 vs. 0.73 ± 0.01) in the adapting bowel. Supplementation of both EGF and VEGF fully rescues adaptation, suggesting that the adaptive response may be dependent on VEGF-driven angiogenesis. These results support a previously unrecognized role for VEGF in the small bowel adaptive response.

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