Role of the LIM class homeodomain protein Xlim-1 in neural and muscle induction by the Spemann organizer in Xenopus

Masanorl Taira, Hiroki Otani, Jean-Pierre Saint-Jeannet, Igor B. Dawid

Research output: Contribution to journalArticle

Abstract

LIKE all known LIM class homeobox genes, Xlim-1 encodes a protein with two tandemly repeated cysteine-rich LIM domains upstream of the homeodomain 1. In Xenopus laevis, Xlim-1 is specifically expressed in the Spemann organizer, whose major functions include neural induction and dorsalization of ventral mesoderm 2-4. From RNA injection experiments we conclude here that: (1) the LIM domains behave as negative regulatory domains; (2) LIM domain mutants of Xlim-1 elicited neural differentiation in animal explants; (3) mutant, and to a lesser extent wild-type, Xlim-1 enhanced muscle formation after coinjection with Xbra; (4) both of these activities are mediated by extracellular signals as seen in combined explant experiments; (5) Xlim-1 mutants activated goosecoid (gsc) expression in animal explants, but not expression of noggin or follistatin; (6) mutant Xlim-1 elicited formation of partial secondary axes, and cooperated with gsc in notochord formation. Thus Xlim-1 has latent activities, implicating it in organizer functions.

Original languageEnglish (US)
Pages (from-to)677-679
Number of pages3
JournalNature
Volume372
Issue number6507
StatePublished - Dec 15 1994

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LIM-Homeodomain Proteins
Embryonic Organizers
Xenopus
Follistatin
Notochord
Muscles
Homeobox Genes
Xenopus laevis
Mesoderm
Cysteine
RNA
Injections
Proteins

ASJC Scopus subject areas

  • General

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Role of the LIM class homeodomain protein Xlim-1 in neural and muscle induction by the Spemann organizer in Xenopus. / Taira, Masanorl; Otani, Hiroki; Saint-Jeannet, Jean-Pierre; Dawid, Igor B.

In: Nature, Vol. 372, No. 6507, 15.12.1994, p. 677-679.

Research output: Contribution to journalArticle

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