Role of Growth Hormone (GH) in liver regeneration

Patricia A. Pennisi, John J. Kopchick, Snorri Thorgeirsson, Derek Leroith, Shoshana Yakar

Research output: Contribution to journalArticle

Abstract

Liver regeneration is a fundamental mechanism by which the liver responds to injury. This process is regulated by endogenous growth factors and cytokines, and it involves proliferation of all mature cells that exist within the intact organ. To understand the role of the GH/IGF-I axis in liver regeneration, we performed partial hepatectomies in three groups of mice: GH antagonist (GHa) transgenic mice, in which the action of GH is blocked; liver IGF-I-deficient mice that lack IGF-I specifically in the liver and also lack the acid-labile subunit (ALS; LID+ALSKO mice), in which IGF-I levels are very low and GH secretion is increased; and control mice. Interestingly, the survival rate of GHa transgenic mice was dramatically reduced after partial hepatectomy (57%) compared with the survival rate of controls (100%) or LID+ALSKO mice (88%). In control mice, the liver was completely regenerated after 4 d, whereas liver regeneration required 7 d in LID+ALSKO mice. In contrast, in GHa mice, liver regeneration reached only 70% of the original liver mass after 4 d and did not improve thereafter. Strikingly, 36 and 48 h after hepatectomy, the livers of control and LID+ALSKO mice, respectively, exhibited intense 5-bromo-2′- deosyuridine (BrdU) staining, whereas BrdU staining was dramatically decreased in the livers of GHa-treated mice. These results suggest that GH plays a critical role in liver regeneration, although whether it acts directly or indirectly remains to be determined.

Original languageEnglish (US)
Pages (from-to)4748-4755
Number of pages8
JournalEndocrinology
Volume145
Issue number10
DOIs
StatePublished - Oct 2004

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Liver Regeneration
Growth Hormone
Insulin-Like Growth Factor I
Liver
Hepatectomy
Transgenic Mice
Staining and Labeling
Hormone Antagonists
Intercellular Signaling Peptides and Proteins
Cytokines

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Pennisi, P. A., Kopchick, J. J., Thorgeirsson, S., Leroith, D., & Yakar, S. (2004). Role of Growth Hormone (GH) in liver regeneration. Endocrinology, 145(10), 4748-4755. https://doi.org/10.1210/en.2004-0655

Role of Growth Hormone (GH) in liver regeneration. / Pennisi, Patricia A.; Kopchick, John J.; Thorgeirsson, Snorri; Leroith, Derek; Yakar, Shoshana.

In: Endocrinology, Vol. 145, No. 10, 10.2004, p. 4748-4755.

Research output: Contribution to journalArticle

Pennisi, PA, Kopchick, JJ, Thorgeirsson, S, Leroith, D & Yakar, S 2004, 'Role of Growth Hormone (GH) in liver regeneration', Endocrinology, vol. 145, no. 10, pp. 4748-4755. https://doi.org/10.1210/en.2004-0655
Pennisi PA, Kopchick JJ, Thorgeirsson S, Leroith D, Yakar S. Role of Growth Hormone (GH) in liver regeneration. Endocrinology. 2004 Oct;145(10):4748-4755. https://doi.org/10.1210/en.2004-0655
Pennisi, Patricia A. ; Kopchick, John J. ; Thorgeirsson, Snorri ; Leroith, Derek ; Yakar, Shoshana. / Role of Growth Hormone (GH) in liver regeneration. In: Endocrinology. 2004 ; Vol. 145, No. 10. pp. 4748-4755.
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