RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II

Mikel Zaratiegui, Stephane E. Castel, Danielle V. Irvine, Anna Kloc, Jie Ren, Fei Li, Elisa De Castro, Laura Marín, An Yun Chang, Derek Goto, W. Zacheus Cande, Francisco Antequera, Benoit Arcangioli, Robert A. Martienssen

Research output: Contribution to journalArticle

Abstract

Heterochromatin comprises tightly compacted repetitive regions of eukaryotic chromosomes. The inheritance of heterochromatin through mitosis requires RNA interference (RNAi), which guides histone modification during the DNA replication phase of the cell cycle. Here we show that the alternating arrangement of origins of replication and non-coding RNA in pericentromeric heterochromatin results in competition between transcription and replication in Schizosaccharomyces pombe. Co-transcriptional RNAi releases RNA polymerase II (Pol II), allowing completion of DNA replication by the leading strand DNA polymerase, and associated histone modifying enzymes that spread heterochromatin with the replication fork. In the absence of RNAi, stalled forks are repaired by homologous recombination without histone modification.

Original languageEnglish (US)
Pages (from-to)135-138
Number of pages4
JournalNature
Volume479
Issue number7371
DOIs
StatePublished - Nov 3 2011

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Heterochromatin
RNA Polymerase II
RNA Interference
Histone Code
DNA Replication
Untranslated RNA
Replication Origin
Schizosaccharomyces
Nucleic Acid Repetitive Sequences
Homologous Recombination
DNA-Directed DNA Polymerase
Mitosis
Histones
Cell Cycle
Chromosomes
Enzymes

ASJC Scopus subject areas

  • General

Cite this

Zaratiegui, M., Castel, S. E., Irvine, D. V., Kloc, A., Ren, J., Li, F., ... Martienssen, R. A. (2011). RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II. Nature, 479(7371), 135-138. https://doi.org/10.1038/nature10501

RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II. / Zaratiegui, Mikel; Castel, Stephane E.; Irvine, Danielle V.; Kloc, Anna; Ren, Jie; Li, Fei; De Castro, Elisa; Marín, Laura; Chang, An Yun; Goto, Derek; Cande, W. Zacheus; Antequera, Francisco; Arcangioli, Benoit; Martienssen, Robert A.

In: Nature, Vol. 479, No. 7371, 03.11.2011, p. 135-138.

Research output: Contribution to journalArticle

Zaratiegui, M, Castel, SE, Irvine, DV, Kloc, A, Ren, J, Li, F, De Castro, E, Marín, L, Chang, AY, Goto, D, Cande, WZ, Antequera, F, Arcangioli, B & Martienssen, RA 2011, 'RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II', Nature, vol. 479, no. 7371, pp. 135-138. https://doi.org/10.1038/nature10501
Zaratiegui M, Castel SE, Irvine DV, Kloc A, Ren J, Li F et al. RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II. Nature. 2011 Nov 3;479(7371):135-138. https://doi.org/10.1038/nature10501
Zaratiegui, Mikel ; Castel, Stephane E. ; Irvine, Danielle V. ; Kloc, Anna ; Ren, Jie ; Li, Fei ; De Castro, Elisa ; Marín, Laura ; Chang, An Yun ; Goto, Derek ; Cande, W. Zacheus ; Antequera, Francisco ; Arcangioli, Benoit ; Martienssen, Robert A. / RNAi promotes heterochromatic silencing through replication-coupled release of RNA Pol II. In: Nature. 2011 ; Vol. 479, No. 7371. pp. 135-138.
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