Rheology of block copolypeptide solutions

Hydrogels with tunable properties

Victor Breedveld, Andrew P. Nowak, Jun Sato, Timothy J. Deming, David J. Pine

Research output: Contribution to journalArticle

Abstract

Amphiphilic block copolypeptides were prepared through transition-metal- mediated polymerization of amino acid N-carboxyanhydrides. In aqueous solution these materials form strong hydrogels at low concentrations. The self-assembly process that is responsible for gelation was investigated by measuring the rheological properties of the gels for a variety of molecular architectures: poly-L-lysine-&-poly-L-leucine diblock and poly-L-lysine-b-poly-L-leucine-b- poly-L-lysine triblock copolypeptides. Experiments showed that the rodlike helical secondary structure of enantionierically pure poly-L-leucine blocks was instrumental for gelation at polypeptide concentrations as low as 0.25 wt %. The hydrophilic polyelectrolyte segments have stretched coil configurations and stabilize the twisted fibril assemblies by forming a corona around the hydrophobic core. The self-assembly of hydrophobic blocks is highly specific and sensitive to the chirality of the helices. It was found that mechanical properties of the gels can be tuned through the molecular architecture of the block copolypeptides and also by carefully mixing different polypeptides in solution.

Original languageEnglish (US)
Pages (from-to)3943-3953
Number of pages11
JournalMacromolecules
Volume37
Issue number10
DOIs
StatePublished - May 18 2004

Fingerprint

Hydrogels
Polypeptides
Gelation
Rheology
Self assembly
Lysine
Gels
Chirality
Polyelectrolytes
Transition metals
Amino acids
Peptides
Polymerization
Mechanical properties
Amino Acids
Experiments
polyleucine

ASJC Scopus subject areas

  • Materials Chemistry

Cite this

Rheology of block copolypeptide solutions : Hydrogels with tunable properties. / Breedveld, Victor; Nowak, Andrew P.; Sato, Jun; Deming, Timothy J.; Pine, David J.

In: Macromolecules, Vol. 37, No. 10, 18.05.2004, p. 3943-3953.

Research output: Contribution to journalArticle

Breedveld, Victor ; Nowak, Andrew P. ; Sato, Jun ; Deming, Timothy J. ; Pine, David J. / Rheology of block copolypeptide solutions : Hydrogels with tunable properties. In: Macromolecules. 2004 ; Vol. 37, No. 10. pp. 3943-3953.
@article{337be071e0e045ca970d1fac2e664136,
title = "Rheology of block copolypeptide solutions: Hydrogels with tunable properties",
abstract = "Amphiphilic block copolypeptides were prepared through transition-metal- mediated polymerization of amino acid N-carboxyanhydrides. In aqueous solution these materials form strong hydrogels at low concentrations. The self-assembly process that is responsible for gelation was investigated by measuring the rheological properties of the gels for a variety of molecular architectures: poly-L-lysine-&-poly-L-leucine diblock and poly-L-lysine-b-poly-L-leucine-b- poly-L-lysine triblock copolypeptides. Experiments showed that the rodlike helical secondary structure of enantionierically pure poly-L-leucine blocks was instrumental for gelation at polypeptide concentrations as low as 0.25 wt {\%}. The hydrophilic polyelectrolyte segments have stretched coil configurations and stabilize the twisted fibril assemblies by forming a corona around the hydrophobic core. The self-assembly of hydrophobic blocks is highly specific and sensitive to the chirality of the helices. It was found that mechanical properties of the gels can be tuned through the molecular architecture of the block copolypeptides and also by carefully mixing different polypeptides in solution.",
author = "Victor Breedveld and Nowak, {Andrew P.} and Jun Sato and Deming, {Timothy J.} and Pine, {David J.}",
year = "2004",
month = "5",
day = "18",
doi = "10.1021/ma049885f",
language = "English (US)",
volume = "37",
pages = "3943--3953",
journal = "Macromolecules",
issn = "0024-9297",
publisher = "American Chemical Society",
number = "10",

}

TY - JOUR

T1 - Rheology of block copolypeptide solutions

T2 - Hydrogels with tunable properties

AU - Breedveld, Victor

AU - Nowak, Andrew P.

AU - Sato, Jun

AU - Deming, Timothy J.

AU - Pine, David J.

PY - 2004/5/18

Y1 - 2004/5/18

N2 - Amphiphilic block copolypeptides were prepared through transition-metal- mediated polymerization of amino acid N-carboxyanhydrides. In aqueous solution these materials form strong hydrogels at low concentrations. The self-assembly process that is responsible for gelation was investigated by measuring the rheological properties of the gels for a variety of molecular architectures: poly-L-lysine-&-poly-L-leucine diblock and poly-L-lysine-b-poly-L-leucine-b- poly-L-lysine triblock copolypeptides. Experiments showed that the rodlike helical secondary structure of enantionierically pure poly-L-leucine blocks was instrumental for gelation at polypeptide concentrations as low as 0.25 wt %. The hydrophilic polyelectrolyte segments have stretched coil configurations and stabilize the twisted fibril assemblies by forming a corona around the hydrophobic core. The self-assembly of hydrophobic blocks is highly specific and sensitive to the chirality of the helices. It was found that mechanical properties of the gels can be tuned through the molecular architecture of the block copolypeptides and also by carefully mixing different polypeptides in solution.

AB - Amphiphilic block copolypeptides were prepared through transition-metal- mediated polymerization of amino acid N-carboxyanhydrides. In aqueous solution these materials form strong hydrogels at low concentrations. The self-assembly process that is responsible for gelation was investigated by measuring the rheological properties of the gels for a variety of molecular architectures: poly-L-lysine-&-poly-L-leucine diblock and poly-L-lysine-b-poly-L-leucine-b- poly-L-lysine triblock copolypeptides. Experiments showed that the rodlike helical secondary structure of enantionierically pure poly-L-leucine blocks was instrumental for gelation at polypeptide concentrations as low as 0.25 wt %. The hydrophilic polyelectrolyte segments have stretched coil configurations and stabilize the twisted fibril assemblies by forming a corona around the hydrophobic core. The self-assembly of hydrophobic blocks is highly specific and sensitive to the chirality of the helices. It was found that mechanical properties of the gels can be tuned through the molecular architecture of the block copolypeptides and also by carefully mixing different polypeptides in solution.

UR - http://www.scopus.com/inward/record.url?scp=2542613793&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2542613793&partnerID=8YFLogxK

U2 - 10.1021/ma049885f

DO - 10.1021/ma049885f

M3 - Article

VL - 37

SP - 3943

EP - 3953

JO - Macromolecules

JF - Macromolecules

SN - 0024-9297

IS - 10

ER -