Reverse engineering of force integration during mitosis in the Drosophila embryo

Roy Wollman, Gul Civelekoglu-Scholey, Jonathan M. Scholey, Alex Mogilner

Research output: Contribution to journalArticle

Abstract

The mitotic spindle is a complex macromolecular machine that coordinates accurate chromosome segregation. The spindle accomplishes its function using forces generated by microtubules (MTs) and multiple molecular motors, but how these forces are integrated remains unclear, since the temporal activation profiles and the mechanical characteristics of the relevant motors are largely unknown. Here, we developed a computational search algorithm that uses experimental measurements to 'reverse engineer' molecular mechanical machines. Our algorithm uses measurements of length time series for wild-type and experimentally perturbed spindles to identify mechanistic models for coordination of the mitotic force generators in Drosophila embryo spindles. The search eliminated thousands of possible models and identified six distinct strategies for MT-motor integration that agree with available data. Many features of these six predicted strategies are conserved, including a persistent kinesin-5-driven sliding filament mechanism combined with the anaphase B-specific inhibition of a kinesin-13 MT depolymerase on spindle poles. Such conserved features allow predictions of force-velocity characteristics and activation-deactivation profiles of key mitotic motors. Identified differences among the six predicted strategies regarding the mechanisms of prometaphase and anaphase spindle elongation suggest future experiments.

Original languageEnglish (US)
Article number195
JournalMolecular systems biology [electronic resource].
Volume4
DOIs
StatePublished - 2008

Fingerprint

Reverse engineering
Reverse Engineering
Drosophilidae
Embryo
Mitosis
Microtubules
mitosis
Drosophila
Kinesin
Anaphase
engineering
Embryonic Structures
microtubules
kinesin
anaphase
Prometaphase
Spindle Poles
Macromolecular Substances
Activation
Chromosome Segregation

Keywords

  • Genetic algorithm
  • Mathematical model
  • Mitosis
  • Reverse engineering
  • Spindle

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Computational Theory and Mathematics
  • Information Systems
  • Applied Mathematics

Cite this

Reverse engineering of force integration during mitosis in the Drosophila embryo. / Wollman, Roy; Civelekoglu-Scholey, Gul; Scholey, Jonathan M.; Mogilner, Alex.

In: Molecular systems biology [electronic resource]., Vol. 4, 195, 2008.

Research output: Contribution to journalArticle

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