Retinoid X receptor (RXR) within the RXR-retinoic acid receptor heterodimer binds its ligand and enhances retinoid-dependent gene expression

Saverio Minucci, Mark Leid, Reiko Toyama, Jean Pierre Saint-Jeannet, Valerie J. Peterson, Valerie Horn, Jane E. Ishmael, Nisan Bhattacharyya, Anup Dey, Igor B. Dawid, Keiko Ozato

Research output: Contribution to journalArticle

Abstract

Retinoic acid receptor (RAR) and retinoid X receptor (RXR) form heterodimers and regulate retinoid-mediated gene expression. We studied binding of RXR- and RAR-selective ligands to the RXR-RAR heterodimer and subsequent transcription. In limited proteolysis analyses, both RXR and RAR in the heterodimer bound their respective ligands and underwent a conformational change in the presence of a retinoic acid-responsive element. In reporter analyses, the RAR ligand (but not the RXR ligand), when added singly, activated transcription, but coaddition of the two ligands led to synergistic activation of transcription. This activation required the AF-2 domain of both RXR and RAR. Genomic footprinting analysis was performed with P19 embryonal carcinoma cells, in which transcription of the RARβ gene is induced upon retinoid addition. Paralleling the reporter activation data, only the RAR ligand induced in vivo occupancy of the RARβ2 promoter when added singly. However, at suboptimal concentrations of RAR ligand, coaddition of the RXR ligand increased the stability of promoter occupancy. Thus, liganded RXR and RAR both participate in transcription. Finally, when these ligands were tested for teratogenic effects on zebra fish and Xenopus embryos, we found that coadministration of the RXR and RAR ligands caused more severe abnormalities in these embryos than either ligand alone, providing biological support fur the synergistic action of the two ligands.

Original languageEnglish (US)
Pages (from-to)644-655
Number of pages12
JournalMolecular and cellular biology
Volume17
Issue number2
DOIs
StatePublished - Feb 1997

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Minucci, S., Leid, M., Toyama, R., Saint-Jeannet, J. P., Peterson, V. J., Horn, V., Ishmael, J. E., Bhattacharyya, N., Dey, A., Dawid, I. B., & Ozato, K. (1997). Retinoid X receptor (RXR) within the RXR-retinoic acid receptor heterodimer binds its ligand and enhances retinoid-dependent gene expression. Molecular and cellular biology, 17(2), 644-655. https://doi.org/10.1128/MCB.17.2.644