Retinoic acid stimulates interstitial collagenase messenger ribonucleic acid in osteosarcoma cells

Thomas J. Connolly, John C. Clohisy, Jeffrey S. Shilt, Kimberly D. Bergman, Nicola Partridge, Cheryl O. Quinn

Research output: Contribution to journalArticle

Abstract

The rat osteoblastic osteosarcoma cell line UMR 106-01 secretes interstitial collagenase in response to retinoic acid (RA). The present study demonstrates by Northern blot analysis that RA causes an increase in collagenase messenger RNA (mRNA) at 6 h, which is maximal at 24 h (20.5 times basal) and declines toward basal level by 72 h. This stimulation is dose dependent, with a maximal response at 5 x 10-7 M RA. Nuclear run- on assays show a greater than 20-fold increase in the rate of collagenase mRNA transcription between 12-24 h after RA treatment. Cycloheximide blocks RA stimulation of collagenase mRNA, demonstrating the need for de novo protein synthesis. RA not only causes an increase in collagenase secretion, but is known to decrease collagen synthesis in UMR 106-01 cells. In this study, the increase in collagenase mRNA is accompanied by a concomitant decrease in the level of α1(I) procollagen mRNA, which is maximal at 24 h (70% decrease), with a return to near-control levels by 72 h. Nuclear run-on assays demonstrated that the decrease in α1(I) procollagen expression does not have a statistically significant transcriptional component. RA did not statistically decrease the stability of α1(I) procollagen mRNA (calculated t( 1/2 ) = 8.06 ± 0.30 and 9.01 ± 0.62 h in the presence and absence of RA, respectively). However, transcription and stability together probably contribute to the major decrease in stable α1(I) procollagen mRNA observed. Cycloheximide treatment inhibits basal level α1(I) procollagen mRNA accumulation, demonstrating the need for on-going protein synthesis to maintain basal expression of this gene.

Original languageEnglish (US)
Pages (from-to)2542-2548
Number of pages7
JournalEndocrinology
Volume135
Issue number6
DOIs
StatePublished - Dec 1994

Fingerprint

Matrix Metalloproteinase 1
Osteosarcoma
Tretinoin
Procollagen
RNA
Collagenases
Messenger RNA
Cycloheximide
Northern Blotting
Proteins
Collagen
Gene Expression
Cell Line

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Connolly, T. J., Clohisy, J. C., Shilt, J. S., Bergman, K. D., Partridge, N., & Quinn, C. O. (1994). Retinoic acid stimulates interstitial collagenase messenger ribonucleic acid in osteosarcoma cells. Endocrinology, 135(6), 2542-2548. https://doi.org/10.1210/en.135.6.2542

Retinoic acid stimulates interstitial collagenase messenger ribonucleic acid in osteosarcoma cells. / Connolly, Thomas J.; Clohisy, John C.; Shilt, Jeffrey S.; Bergman, Kimberly D.; Partridge, Nicola; Quinn, Cheryl O.

In: Endocrinology, Vol. 135, No. 6, 12.1994, p. 2542-2548.

Research output: Contribution to journalArticle

Connolly, Thomas J. ; Clohisy, John C. ; Shilt, Jeffrey S. ; Bergman, Kimberly D. ; Partridge, Nicola ; Quinn, Cheryl O. / Retinoic acid stimulates interstitial collagenase messenger ribonucleic acid in osteosarcoma cells. In: Endocrinology. 1994 ; Vol. 135, No. 6. pp. 2542-2548.
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abstract = "The rat osteoblastic osteosarcoma cell line UMR 106-01 secretes interstitial collagenase in response to retinoic acid (RA). The present study demonstrates by Northern blot analysis that RA causes an increase in collagenase messenger RNA (mRNA) at 6 h, which is maximal at 24 h (20.5 times basal) and declines toward basal level by 72 h. This stimulation is dose dependent, with a maximal response at 5 x 10-7 M RA. Nuclear run- on assays show a greater than 20-fold increase in the rate of collagenase mRNA transcription between 12-24 h after RA treatment. Cycloheximide blocks RA stimulation of collagenase mRNA, demonstrating the need for de novo protein synthesis. RA not only causes an increase in collagenase secretion, but is known to decrease collagen synthesis in UMR 106-01 cells. In this study, the increase in collagenase mRNA is accompanied by a concomitant decrease in the level of α1(I) procollagen mRNA, which is maximal at 24 h (70{\%} decrease), with a return to near-control levels by 72 h. Nuclear run-on assays demonstrated that the decrease in α1(I) procollagen expression does not have a statistically significant transcriptional component. RA did not statistically decrease the stability of α1(I) procollagen mRNA (calculated t( 1/2 ) = 8.06 ± 0.30 and 9.01 ± 0.62 h in the presence and absence of RA, respectively). However, transcription and stability together probably contribute to the major decrease in stable α1(I) procollagen mRNA observed. Cycloheximide treatment inhibits basal level α1(I) procollagen mRNA accumulation, demonstrating the need for on-going protein synthesis to maintain basal expression of this gene.",
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