Rescue of Inhibitory Synapse Strength following Developmental Hearing Loss

Vibhakar C. Kotak, Anne E. Takesian, Patricia C. MacKenzie, Dan Sanes

Research output: Contribution to journalArticle

Abstract

Inhibitory synapse dysfunction may contribute to many developmental brain disorders, including the secondary consequences of sensory deprivation. In fact, developmental hearing loss leads to a profound reduction in the strength of inhibitory postsynaptic currents (IPSCs) in the auditory cortex, and this deficit persists into adulthood. This finding is consistent with the general theory that the emergence of mature synaptic properties requires activity during development. Therefore, we tested the prediction that inhibitory strength can be restored following developmental hearing loss by boosting GABAergic transmission in vivo. Conductive or sensorineural hearing loss was induced surgically in gerbils prior to hearing onset and GABA agonists were then administered for one week. IPSCs were subsequently recorded from pyramidal neurons in a thalamocortical brain slice preparation. Administration of either a GABAA receptor a1 subunit specific agonist (zolpidem), or a selective GABA reuptake inhibitor (SGRI), rescued IPSC amplitude in hearing loss animals. Furthermore, this restoration persisted in adults, long after drug treatment ended. In contrast, a GABAB receptor agonist baclofen did not restore inhibitory strength. IPSCs could also be restored when SGRI administration began 3 weeks after sensory deprivation. Together, these results demonstrate long-lasting restoration of cortical inhibitory strength in the absence of normal experience. This suggests that in vivo GABAA receptor activation is sufficient to promote maturation, and this principle may extend to other developmental disorders associated with diminished inhibitory function.

Original languageEnglish (US)
Article numbere53438
JournalPLoS One
Volume8
Issue number1
DOIs
StatePublished - Jan 17 2013

Fingerprint

Inhibitory Postsynaptic Potentials
Audition
hearing
synapse
Hearing Loss
Synapses
GABA Uptake Inhibitors
Sensory Deprivation
agonists
GABA-A Receptors
receptors
Restoration
GABA-B Receptors
GABA Agonists
Conductive Hearing Loss
Brain
Auditory Cortex
Gerbillinae
Sensorineural Hearing Loss
Pyramidal Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Rescue of Inhibitory Synapse Strength following Developmental Hearing Loss. / Kotak, Vibhakar C.; Takesian, Anne E.; MacKenzie, Patricia C.; Sanes, Dan.

In: PLoS One, Vol. 8, No. 1, e53438, 17.01.2013.

Research output: Contribution to journalArticle

Kotak, Vibhakar C. ; Takesian, Anne E. ; MacKenzie, Patricia C. ; Sanes, Dan. / Rescue of Inhibitory Synapse Strength following Developmental Hearing Loss. In: PLoS One. 2013 ; Vol. 8, No. 1.
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