Repeatability of in vivo 3D lamina cribrosa microarchitecture using adaptive optics spectral domain optical coherence tomography

Zach Nadler, Bo Wang, Gadi Wollstein, Jessica E. Nevins, Hiroshi Ishikawa, Richard Bilonick, Larry Kagemann, Ian A. Sigal, R. Daniel Ferguson, Ankit Patel, Daniel X. Hammer, Joel S. Schuman

    Research output: Contribution to journalArticle

    Abstract

    We demonstrate the repeatability of lamina cribrosa (LC) microarchitecture for in vivo 3D optical coherence tomography (OCT) scans of healthy, glaucoma suspects, and glaucomatous eyes. Eyes underwent two scans using a prototype adaptive optics spectral domain OCT (AO-SDOCT) device from which LC microarchitecture was semiautomatically segmented. LC segmentations were used to quantify pore and beam structure through several global microarchitecture parameters. Repeatability of LC microarchitecture was assessed qualitatively and quantitatively by calculating parameter imprecision. For all but one parameters (pore volume) measurement imprecision was <4.7% of the mean value, indicating good measurement reproducibility. Imprecision ranged between 27.3% and 54.5% of the population standard deviation for each parameter, while there was not a significant effect on imprecision due to disease status, indicating utility in testing for LC structural trends.

    Original languageEnglish (US)
    Pages (from-to)1114-1123
    Number of pages10
    JournalBiomedical Optics Express
    Volume5
    Issue number4
    DOIs
    StatePublished - Apr 1 2014

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    ASJC Scopus subject areas

    • Biotechnology
    • Atomic and Molecular Physics, and Optics

    Cite this

    Nadler, Z., Wang, B., Wollstein, G., Nevins, J. E., Ishikawa, H., Bilonick, R., Kagemann, L., Sigal, I. A., Ferguson, R. D., Patel, A., Hammer, D. X., & Schuman, J. S. (2014). Repeatability of in vivo 3D lamina cribrosa microarchitecture using adaptive optics spectral domain optical coherence tomography. Biomedical Optics Express, 5(4), 1114-1123. https://doi.org/10.1364/BOE.5.001114