Release of the recombinant proteins, human serum albumin, β-glucuronidase, glycoprotein B from human cytomegalovirus, and green fluorescent protein, in root exudates from transgenic tobacco and their effects on microbes and enzymatic activities in soil

N. Sabharwal, I. Icoz, Deepak Saxena, G. Stotzky

Research output: Contribution to journalArticle

Abstract

We determined the release in root exudates of human serum albumin (HSA), β-glucuronidase (GUS), glycoprotein B (gB) from human cytomegalovirus, and green fluorescent protein (GFP) from genetically modified transgenic tobacco expressing the genes for these proteins in hydroponic culture and non-sterile soil. GUS, gB, and GFP were expressed in the plant but were not released in root exudates, whereas HSA was both expressed in the plant and released in root exudates, as shown by a 66.5-kDa band on SDS-PAGE and Western blot and confirmed by ELISA. Root exudates from GUS and gB plants showed no bands that could be attributed to these proteins on SDS-PAGE, and root exudates from GFP plants showed no fluorescence. The concentration of HSA in root exudates was estimated to be 0.021 ng ml-1, whereas that in the plant biomass was estimated to be 0.087 ng ml-1. The concentration of HSA in soil was estimated to be 0.049 ng g-1. No significant differences in the number of microorganisms and the activity of selected enzymes were observed between rhizosphere soil of non-modified and HSA tobacco.

Original languageEnglish (US)
Pages (from-to)464-469
Number of pages6
JournalPlant Physiology and Biochemistry
Volume45
Issue number6-7
DOIs
StatePublished - Jun 2007

Fingerprint

Human herpesvirus 5
Tobacco
Glucuronidase
root exudates
Exudates and Transudates
Green Fluorescent Proteins
green fluorescent protein
Recombinant Proteins
recombinant proteins
Serum Albumin
glycoproteins
Glycoproteins
tobacco
Soil
genetically modified organisms
microorganisms
Soils
soil
Polyacrylamide Gel Electrophoresis
Hydroponics

Keywords

  • β-Glucuronidase
  • Glycoprotein B
  • Green fluorescent protein
  • Human serum albumin
  • Root exudates
  • Soil enzymes
  • Soil microbes
  • Transgenic tobacco

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

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title = "Release of the recombinant proteins, human serum albumin, β-glucuronidase, glycoprotein B from human cytomegalovirus, and green fluorescent protein, in root exudates from transgenic tobacco and their effects on microbes and enzymatic activities in soil",
abstract = "We determined the release in root exudates of human serum albumin (HSA), β-glucuronidase (GUS), glycoprotein B (gB) from human cytomegalovirus, and green fluorescent protein (GFP) from genetically modified transgenic tobacco expressing the genes for these proteins in hydroponic culture and non-sterile soil. GUS, gB, and GFP were expressed in the plant but were not released in root exudates, whereas HSA was both expressed in the plant and released in root exudates, as shown by a 66.5-kDa band on SDS-PAGE and Western blot and confirmed by ELISA. Root exudates from GUS and gB plants showed no bands that could be attributed to these proteins on SDS-PAGE, and root exudates from GFP plants showed no fluorescence. The concentration of HSA in root exudates was estimated to be 0.021 ng ml-1, whereas that in the plant biomass was estimated to be 0.087 ng ml-1. The concentration of HSA in soil was estimated to be 0.049 ng g-1. No significant differences in the number of microorganisms and the activity of selected enzymes were observed between rhizosphere soil of non-modified and HSA tobacco.",
keywords = "β-Glucuronidase, Glycoprotein B, Green fluorescent protein, Human serum albumin, Root exudates, Soil enzymes, Soil microbes, Transgenic tobacco",
author = "N. Sabharwal and I. Icoz and Deepak Saxena and G. Stotzky",
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TY - JOUR

T1 - Release of the recombinant proteins, human serum albumin, β-glucuronidase, glycoprotein B from human cytomegalovirus, and green fluorescent protein, in root exudates from transgenic tobacco and their effects on microbes and enzymatic activities in soil

AU - Sabharwal, N.

AU - Icoz, I.

AU - Saxena, Deepak

AU - Stotzky, G.

PY - 2007/6

Y1 - 2007/6

N2 - We determined the release in root exudates of human serum albumin (HSA), β-glucuronidase (GUS), glycoprotein B (gB) from human cytomegalovirus, and green fluorescent protein (GFP) from genetically modified transgenic tobacco expressing the genes for these proteins in hydroponic culture and non-sterile soil. GUS, gB, and GFP were expressed in the plant but were not released in root exudates, whereas HSA was both expressed in the plant and released in root exudates, as shown by a 66.5-kDa band on SDS-PAGE and Western blot and confirmed by ELISA. Root exudates from GUS and gB plants showed no bands that could be attributed to these proteins on SDS-PAGE, and root exudates from GFP plants showed no fluorescence. The concentration of HSA in root exudates was estimated to be 0.021 ng ml-1, whereas that in the plant biomass was estimated to be 0.087 ng ml-1. The concentration of HSA in soil was estimated to be 0.049 ng g-1. No significant differences in the number of microorganisms and the activity of selected enzymes were observed between rhizosphere soil of non-modified and HSA tobacco.

AB - We determined the release in root exudates of human serum albumin (HSA), β-glucuronidase (GUS), glycoprotein B (gB) from human cytomegalovirus, and green fluorescent protein (GFP) from genetically modified transgenic tobacco expressing the genes for these proteins in hydroponic culture and non-sterile soil. GUS, gB, and GFP were expressed in the plant but were not released in root exudates, whereas HSA was both expressed in the plant and released in root exudates, as shown by a 66.5-kDa band on SDS-PAGE and Western blot and confirmed by ELISA. Root exudates from GUS and gB plants showed no bands that could be attributed to these proteins on SDS-PAGE, and root exudates from GFP plants showed no fluorescence. The concentration of HSA in root exudates was estimated to be 0.021 ng ml-1, whereas that in the plant biomass was estimated to be 0.087 ng ml-1. The concentration of HSA in soil was estimated to be 0.049 ng g-1. No significant differences in the number of microorganisms and the activity of selected enzymes were observed between rhizosphere soil of non-modified and HSA tobacco.

KW - β-Glucuronidase

KW - Glycoprotein B

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KW - Human serum albumin

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KW - Soil enzymes

KW - Soil microbes

KW - Transgenic tobacco

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