Relaxin does not rescue coronal suture fusion in craniosynostotic rabbits

James J. Cray, Anne M. Burrows, Lisa Vecchione, Christopher R. Kinsella, Joseph E. Losee, Amr Moursi, Michael I. Siegel, Gregory M. Cooper, Mark P. Mooney

Research output: Contribution to journalArticle

Abstract

Objectives: Craniosynostosis affects 1 in 2000 to 3000 live births and may result in craniofacial and neural growth disturbances. Histological data have shown that thick collagenous bundles are present in the sutural ligament, which may tether the osteogenic fronts, resulting in premature fusion. The hormone relaxin has been shown to disrupt collagen fiber organization, possibly preventing craniosynostosis by relaxing the sutural ligament and allowing osteogenic fronts to separate normally and stay patent. This study tested this hypothesis with a rabbit model of delayed-onset coronal suture synostosis. Methods: A total of 18 New Zealand White rabbits with craniosynostosis were randomly assigned to one of three groups: sham control, protein control (BSA), relaxin treatment. After initial diagnosis, sham surgery, BSA, or relaxin was delivered to the fusing coronal suture in a slow-release (56-day) collagen vehicle. Longitudinal radiographs and body weights were collected at 10, 25, 42, and 84 days of age, and sutures were harvested for histology. Results: Relaxin-treated animals had more disorganized intrasuture content than control groups. These specimens also appeared to have relatively wider sutures ectocranially. There were no significant differences in relaxin-treated animals for all craniofacial growth measures, or suture separation compared with controls. Conclusions: These data do not support our initial hypothesis that the use of relaxin may rescue sutures destined to undergo premature suture fusion. These findings suggest that collagen fiber arrangement may not be important for suture fusion. This protein therapy would not be clinically useful for craniosynostosis.

Original languageEnglish (US)
JournalCleft Palate-Craniofacial Journal
Volume49
Issue number5
DOIs
StatePublished - Sep 2012

Fingerprint

Relaxin
Sutures
Rabbits
Craniosynostoses
Collagen
Ligaments
Synostosis
Control Groups
Live Birth
Growth
Histology
Proteins
Body Weight
Hormones

Keywords

  • Cephalometrics
  • Collagen fibers
  • Craniosynostosis
  • Extracellularmatrix
  • Relaxin

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Oral Surgery

Cite this

Cray, J. J., Burrows, A. M., Vecchione, L., Kinsella, C. R., Losee, J. E., Moursi, A., ... Mooney, M. P. (2012). Relaxin does not rescue coronal suture fusion in craniosynostotic rabbits. Cleft Palate-Craniofacial Journal, 49(5). https://doi.org/10.1597/11-024

Relaxin does not rescue coronal suture fusion in craniosynostotic rabbits. / Cray, James J.; Burrows, Anne M.; Vecchione, Lisa; Kinsella, Christopher R.; Losee, Joseph E.; Moursi, Amr; Siegel, Michael I.; Cooper, Gregory M.; Mooney, Mark P.

In: Cleft Palate-Craniofacial Journal, Vol. 49, No. 5, 09.2012.

Research output: Contribution to journalArticle

Cray, JJ, Burrows, AM, Vecchione, L, Kinsella, CR, Losee, JE, Moursi, A, Siegel, MI, Cooper, GM & Mooney, MP 2012, 'Relaxin does not rescue coronal suture fusion in craniosynostotic rabbits', Cleft Palate-Craniofacial Journal, vol. 49, no. 5. https://doi.org/10.1597/11-024
Cray, James J. ; Burrows, Anne M. ; Vecchione, Lisa ; Kinsella, Christopher R. ; Losee, Joseph E. ; Moursi, Amr ; Siegel, Michael I. ; Cooper, Gregory M. ; Mooney, Mark P. / Relaxin does not rescue coronal suture fusion in craniosynostotic rabbits. In: Cleft Palate-Craniofacial Journal. 2012 ; Vol. 49, No. 5.
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