Regulation of iron transport related genes by boron in the marine bacterium Marinobacter algicola DG893

Ariel Romano, Lyndsay Trimble, Ashtian R. Hobusch, Kristine J. Schroeder, Shady Amin, Andrej D. Hartnett, Ryan A. Barker, Alvin L. Crumbliss, Carl J. Carrano

    Research output: Contribution to journalArticle

    Abstract

    While there has been extensive interest in the use of boron isotope ratios as a surrogate of pH in paleoclimate studies in the context of climate change-related questions, the high (0.4 mM) concentration and the depth-independent (conservative or non-nutrient-like) concentration profile of this element have led to boron being neglected as a potentially biologically relevant element in the modern ocean. Here we report that boron affects the expression of a number of protein and genes in the "algal-associated" Gram-negative marine bacterium Marinobacter algicola DG893. Most intriguingly, a number of these proteins and genes are related to iron uptake. In a recent separate publication we have shown that boron regulates one such iron transport related protein, i.e. the periplasmic iron binding protein FbpA via a direct interaction of the metalloid with this protein. Here we show that a number of other iron uptake related genes are also affected by boron but in the opposite way i.e. they are up-regulated. We propose that the differential effect of boron on FbpA expression relative to other iron transport related genes is a result of an interaction between boron and the global iron regulatory protein Fur.

    Original languageEnglish (US)
    Pages (from-to)1025-1030
    Number of pages6
    JournalMetallomics
    Volume5
    Issue number8
    DOIs
    StatePublished - Aug 1 2013

    Fingerprint

    Marinobacter
    Boron
    Bacteria
    Iron
    Genes
    Proteins
    Algal Proteins
    Iron-Regulatory Proteins
    Periplasmic Binding Proteins
    Iron-Binding Proteins
    Metalloids
    Climate Change
    Gram-Negative Bacteria
    Climate change
    Oceans and Seas
    Isotopes
    Publications
    Carrier Proteins

    ASJC Scopus subject areas

    • Chemistry (miscellaneous)
    • Biophysics
    • Biochemistry
    • Biomaterials
    • Metals and Alloys

    Cite this

    Romano, A., Trimble, L., Hobusch, A. R., Schroeder, K. J., Amin, S., Hartnett, A. D., ... Carrano, C. J. (2013). Regulation of iron transport related genes by boron in the marine bacterium Marinobacter algicola DG893. Metallomics, 5(8), 1025-1030. https://doi.org/10.1039/c3mt00068k

    Regulation of iron transport related genes by boron in the marine bacterium Marinobacter algicola DG893. / Romano, Ariel; Trimble, Lyndsay; Hobusch, Ashtian R.; Schroeder, Kristine J.; Amin, Shady; Hartnett, Andrej D.; Barker, Ryan A.; Crumbliss, Alvin L.; Carrano, Carl J.

    In: Metallomics, Vol. 5, No. 8, 01.08.2013, p. 1025-1030.

    Research output: Contribution to journalArticle

    Romano, A, Trimble, L, Hobusch, AR, Schroeder, KJ, Amin, S, Hartnett, AD, Barker, RA, Crumbliss, AL & Carrano, CJ 2013, 'Regulation of iron transport related genes by boron in the marine bacterium Marinobacter algicola DG893', Metallomics, vol. 5, no. 8, pp. 1025-1030. https://doi.org/10.1039/c3mt00068k
    Romano A, Trimble L, Hobusch AR, Schroeder KJ, Amin S, Hartnett AD et al. Regulation of iron transport related genes by boron in the marine bacterium Marinobacter algicola DG893. Metallomics. 2013 Aug 1;5(8):1025-1030. https://doi.org/10.1039/c3mt00068k
    Romano, Ariel ; Trimble, Lyndsay ; Hobusch, Ashtian R. ; Schroeder, Kristine J. ; Amin, Shady ; Hartnett, Andrej D. ; Barker, Ryan A. ; Crumbliss, Alvin L. ; Carrano, Carl J. / Regulation of iron transport related genes by boron in the marine bacterium Marinobacter algicola DG893. In: Metallomics. 2013 ; Vol. 5, No. 8. pp. 1025-1030.
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