Regulation of iron transport related genes by boron in the marine bacterium Marinobacter algicola DG893

Ariel Romano, Lyndsay Trimble, Ashtian R. Hobusch, Kristine J. Schroeder, Shady Amin, Andrej D. Hartnett, Ryan A. Barker, Alvin L. Crumbliss, Carl J. Carrano

Research output: Contribution to journalArticle

Abstract

While there has been extensive interest in the use of boron isotope ratios as a surrogate of pH in paleoclimate studies in the context of climate change-related questions, the high (0.4 mM) concentration and the depth-independent (conservative or non-nutrient-like) concentration profile of this element have led to boron being neglected as a potentially biologically relevant element in the modern ocean. Here we report that boron affects the expression of a number of protein and genes in the "algal-associated" Gram-negative marine bacterium Marinobacter algicola DG893. Most intriguingly, a number of these proteins and genes are related to iron uptake. In a recent separate publication we have shown that boron regulates one such iron transport related protein, i.e. the periplasmic iron binding protein FbpA via a direct interaction of the metalloid with this protein. Here we show that a number of other iron uptake related genes are also affected by boron but in the opposite way i.e. they are up-regulated. We propose that the differential effect of boron on FbpA expression relative to other iron transport related genes is a result of an interaction between boron and the global iron regulatory protein Fur.

Original languageEnglish (US)
Pages (from-to)1025-1030
Number of pages6
JournalMetallomics
Volume5
Issue number8
DOIs
StatePublished - Aug 1 2013

Fingerprint

Marinobacter
Boron
Bacteria
Iron
Genes
Proteins
Algal Proteins
Iron-Regulatory Proteins
Periplasmic Binding Proteins
Iron-Binding Proteins
Metalloids
Climate Change
Gram-Negative Bacteria
Climate change
Oceans and Seas
Isotopes
Publications
Carrier Proteins

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Biophysics
  • Biochemistry
  • Biomaterials
  • Metals and Alloys

Cite this

Romano, A., Trimble, L., Hobusch, A. R., Schroeder, K. J., Amin, S., Hartnett, A. D., ... Carrano, C. J. (2013). Regulation of iron transport related genes by boron in the marine bacterium Marinobacter algicola DG893. Metallomics, 5(8), 1025-1030. https://doi.org/10.1039/c3mt00068k

Regulation of iron transport related genes by boron in the marine bacterium Marinobacter algicola DG893. / Romano, Ariel; Trimble, Lyndsay; Hobusch, Ashtian R.; Schroeder, Kristine J.; Amin, Shady; Hartnett, Andrej D.; Barker, Ryan A.; Crumbliss, Alvin L.; Carrano, Carl J.

In: Metallomics, Vol. 5, No. 8, 01.08.2013, p. 1025-1030.

Research output: Contribution to journalArticle

Romano, A, Trimble, L, Hobusch, AR, Schroeder, KJ, Amin, S, Hartnett, AD, Barker, RA, Crumbliss, AL & Carrano, CJ 2013, 'Regulation of iron transport related genes by boron in the marine bacterium Marinobacter algicola DG893', Metallomics, vol. 5, no. 8, pp. 1025-1030. https://doi.org/10.1039/c3mt00068k
Romano, Ariel ; Trimble, Lyndsay ; Hobusch, Ashtian R. ; Schroeder, Kristine J. ; Amin, Shady ; Hartnett, Andrej D. ; Barker, Ryan A. ; Crumbliss, Alvin L. ; Carrano, Carl J. / Regulation of iron transport related genes by boron in the marine bacterium Marinobacter algicola DG893. In: Metallomics. 2013 ; Vol. 5, No. 8. pp. 1025-1030.
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