Regional distribution of mineral and matrix in the femurs of rats flown on Cosmos 1887 biosatellite

Gerald L. Mechanic, Sara B. Arnaud, Alan Boyde, Timothy Bromage, Patricia Buckendahl, James C. Elliott, Elton P. Katz, Galina N. Durnova

Research output: Contribution to journalArticle

Abstract

We combined biochemical measurements with novel techniques for image analysis in the rat femur to characterize the location and nature of the defect in mineralization known to occur in growing animals after spaceflight. Concentrations of mineral and osteocalcin were low in the distal half of the diaphysis and concentrations of collagen were low with evidence of increased synthesis in the proximal half of the diaphysis of the flight bones. X-ray microtomography provided semiquantitative data in computer-generated sections of whole wet bone that indicated a longitudinal gradient of decreasing mineralization toward the distal diaphysis, similar to the chemistry results. Analysis of embedded sections by backscattered electrons in a scanning electron microscope revealed distinct patterns of mineral distribution in the proximal, central, and distal regions of the diaphysis and also showed a net reduction in mineral levels toward the distal shaft. Increases in mineral density to higher fractions in controls were less in the flight bones at all three levels, with the most distal cross-sectional area most affected. The combined results from these novel techniques identified the areas of femoral diaphysis most vulnerable to the mineralization defect associated with spaceflight and/or the stress of landing.

Original languageEnglish (US)
Pages (from-to)34-40
Number of pages7
JournalFASEB Journal
Volume4
Issue number1
StatePublished - 1990

Fingerprint

Spacecraft
Cosmos
Diaphyses
femur
Femur
Minerals
Rats
space flight
mineralization
bones
minerals
Bone
rats
flight
Space Flight
micro-computed tomography
osteocalcin
Bone and Bones
thighs
scanning electron microscopes

Keywords

  • Backscattered electron imaging
  • Biochemistry
  • Microtomography
  • Rat femur
  • Spaceflight

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Mechanic, G. L., Arnaud, S. B., Boyde, A., Bromage, T., Buckendahl, P., Elliott, J. C., ... Durnova, G. N. (1990). Regional distribution of mineral and matrix in the femurs of rats flown on Cosmos 1887 biosatellite. FASEB Journal, 4(1), 34-40.

Regional distribution of mineral and matrix in the femurs of rats flown on Cosmos 1887 biosatellite. / Mechanic, Gerald L.; Arnaud, Sara B.; Boyde, Alan; Bromage, Timothy; Buckendahl, Patricia; Elliott, James C.; Katz, Elton P.; Durnova, Galina N.

In: FASEB Journal, Vol. 4, No. 1, 1990, p. 34-40.

Research output: Contribution to journalArticle

Mechanic, GL, Arnaud, SB, Boyde, A, Bromage, T, Buckendahl, P, Elliott, JC, Katz, EP & Durnova, GN 1990, 'Regional distribution of mineral and matrix in the femurs of rats flown on Cosmos 1887 biosatellite', FASEB Journal, vol. 4, no. 1, pp. 34-40.
Mechanic GL, Arnaud SB, Boyde A, Bromage T, Buckendahl P, Elliott JC et al. Regional distribution of mineral and matrix in the femurs of rats flown on Cosmos 1887 biosatellite. FASEB Journal. 1990;4(1):34-40.
Mechanic, Gerald L. ; Arnaud, Sara B. ; Boyde, Alan ; Bromage, Timothy ; Buckendahl, Patricia ; Elliott, James C. ; Katz, Elton P. ; Durnova, Galina N. / Regional distribution of mineral and matrix in the femurs of rats flown on Cosmos 1887 biosatellite. In: FASEB Journal. 1990 ; Vol. 4, No. 1. pp. 34-40.
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