Regeneration of the cementum and periodontal ligament using local BDNF delivery in class II furcation defects

Ryo Jimbo, Jessica Singer, Nick Tovar, Charles Marin, Rodrigo Neiva, Estevam A. Bonfante, Malvin N. Janal, Hugues Contamin, Paulo Coelho

Research output: Contribution to journalArticle

Abstract

Periodontal furcation defects are usually addressed by the placement of a physical barrier which may limit the regenerative potential of periodontal wounds. This study morphometrically quantified the regenerative effect of brain-derived neurotrophic factor (BDNF) in furcation defects in a non-human primate model. Grade II furcation defects (with and without induced inflammation prior to surgery) were created on the first and second molars of eight non-human primates. Defects were treated with open flap debridement and subsequently filled with either: Group A; BDNF (500 μg mL-1) in high-molecular weight-hyaluronic acid (HMW-HA), Group B; BDNF (50 μg mL-1) in HMW-HA, Group C; HMW-HA acid only, Group D; unfilled defect, or Group E; BDNF (500 μg mL-1) in saline. Periodontal wound healing was observed every 2 weeks by computed-tomography. At 11 weeks all animals were sacrificed and maxillary and mandibular block biopsies were referred for nondecalcified histology. Linear measurements of new cementum (cellular and acellular) and periodontal ligament (PDL) formation were performed. Computerized-tomography reconstruction and software quantification demonstrated successful bone fill for all groups. However, histometric assessment demonstrated significantly higher level of total periodontal regeneration for the 500 μg mL-1 BDNF HMW-HA relative to all other groups. No significant differences in cementogenesis were observed among groups. Significantly higher acellular cementum formation was observed for sites where inflammation was not induced prior to surgical procedures. While all groups experienced similar bone fill and cementogenesis, the 500 μg mL-1 BDNF HMW-HA appeared to most effectively repair PDL (minimum increase of ∼22% relative to all groups; over 200% relative to unfilled defects).

Original languageEnglish (US)
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
DOIs
StateAccepted/In press - 2017

Fingerprint

Ligaments
Brain-Derived Neurotrophic Factor
Hyaluronic acid
Hyaluronic Acid
Brain
Molecular weight
Defects
Bone
Histology
Computerized tomography
Biopsy
Surgery
Tomography
Nerve Growth Factors
Animals
Repair
Acids

Keywords

  • BDNF
  • Furcation
  • Histomorphometry
  • Hyaluronic acid
  • Periodontal regeneration

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Regeneration of the cementum and periodontal ligament using local BDNF delivery in class II furcation defects. / Jimbo, Ryo; Singer, Jessica; Tovar, Nick; Marin, Charles; Neiva, Rodrigo; Bonfante, Estevam A.; Janal, Malvin N.; Contamin, Hugues; Coelho, Paulo.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, 2017.

Research output: Contribution to journalArticle

Jimbo, Ryo ; Singer, Jessica ; Tovar, Nick ; Marin, Charles ; Neiva, Rodrigo ; Bonfante, Estevam A. ; Janal, Malvin N. ; Contamin, Hugues ; Coelho, Paulo. / Regeneration of the cementum and periodontal ligament using local BDNF delivery in class II furcation defects. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2017.
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