Reduced sympathetic innervation after alteration of target cell neurotransmitter phenotype in transgenic mice

Sunghee Cho, Jin H. Son, Dong H. Park, Chiye Aoki, Xiuzhen Song, Gerard P. Smith, Tong H. Joh

Research output: Contribution to journalArticle

Abstract

Neurotransmitters play a variety of important roles during nervous system development. In the present study, we hypothesized that neurotransmitter phenotype of both projecting and target cells is an important factor for the final synaptic linkage and its specificity. To test this hypothesis, we used transgenic techniques to convert serotonin/melatonin-producing cells of the pineal gland into cells that also produce dopamine and investigated the innervation of the phenotypically altered target cells. This phenotypic alteration markedly reduced the noradrenergic innervation originating from the superior cervical ganglia. Although the mechanism by which the reduction occurs is presently unknown, quantitative enzyme-linked immunoassay showed the presence of the equivalent amounts of nerve growth factor (NGF) in the control and transgenic pineal glands, suggesting that it occurred in a NGF- independent manner. The results suggest that target neurotransmitter phenotype influences the formation of afferent connections during development.

Original languageEnglish (US)
Pages (from-to)2862-2866
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number7
DOIs
StatePublished - Apr 2 1996

Fingerprint

Transgenic Mice
Neurotransmitter Agents
Phenotype
Pineal Gland
Nerve Growth Factor
Superior Cervical Ganglion
Melatonin
Immunoenzyme Techniques
Nervous System
Dopamine
Serotonin

Keywords

  • dopamine
  • pineal gland
  • superior cervical ganglion
  • tyrosine hydroxylase

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Reduced sympathetic innervation after alteration of target cell neurotransmitter phenotype in transgenic mice. / Cho, Sunghee; Son, Jin H.; Park, Dong H.; Aoki, Chiye; Song, Xiuzhen; Smith, Gerard P.; Joh, Tong H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 7, 02.04.1996, p. 2862-2866.

Research output: Contribution to journalArticle

Cho, Sunghee ; Son, Jin H. ; Park, Dong H. ; Aoki, Chiye ; Song, Xiuzhen ; Smith, Gerard P. ; Joh, Tong H. / Reduced sympathetic innervation after alteration of target cell neurotransmitter phenotype in transgenic mice. In: Proceedings of the National Academy of Sciences of the United States of America. 1996 ; Vol. 93, No. 7. pp. 2862-2866.
@article{e571942c3a48460881b1e9ab79c67486,
title = "Reduced sympathetic innervation after alteration of target cell neurotransmitter phenotype in transgenic mice",
abstract = "Neurotransmitters play a variety of important roles during nervous system development. In the present study, we hypothesized that neurotransmitter phenotype of both projecting and target cells is an important factor for the final synaptic linkage and its specificity. To test this hypothesis, we used transgenic techniques to convert serotonin/melatonin-producing cells of the pineal gland into cells that also produce dopamine and investigated the innervation of the phenotypically altered target cells. This phenotypic alteration markedly reduced the noradrenergic innervation originating from the superior cervical ganglia. Although the mechanism by which the reduction occurs is presently unknown, quantitative enzyme-linked immunoassay showed the presence of the equivalent amounts of nerve growth factor (NGF) in the control and transgenic pineal glands, suggesting that it occurred in a NGF- independent manner. The results suggest that target neurotransmitter phenotype influences the formation of afferent connections during development.",
keywords = "dopamine, pineal gland, superior cervical ganglion, tyrosine hydroxylase",
author = "Sunghee Cho and Son, {Jin H.} and Park, {Dong H.} and Chiye Aoki and Xiuzhen Song and Smith, {Gerard P.} and Joh, {Tong H.}",
year = "1996",
month = "4",
day = "2",
doi = "10.1073/pnas.93.7.2862",
language = "English (US)",
volume = "93",
pages = "2862--2866",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "7",

}

TY - JOUR

T1 - Reduced sympathetic innervation after alteration of target cell neurotransmitter phenotype in transgenic mice

AU - Cho, Sunghee

AU - Son, Jin H.

AU - Park, Dong H.

AU - Aoki, Chiye

AU - Song, Xiuzhen

AU - Smith, Gerard P.

AU - Joh, Tong H.

PY - 1996/4/2

Y1 - 1996/4/2

N2 - Neurotransmitters play a variety of important roles during nervous system development. In the present study, we hypothesized that neurotransmitter phenotype of both projecting and target cells is an important factor for the final synaptic linkage and its specificity. To test this hypothesis, we used transgenic techniques to convert serotonin/melatonin-producing cells of the pineal gland into cells that also produce dopamine and investigated the innervation of the phenotypically altered target cells. This phenotypic alteration markedly reduced the noradrenergic innervation originating from the superior cervical ganglia. Although the mechanism by which the reduction occurs is presently unknown, quantitative enzyme-linked immunoassay showed the presence of the equivalent amounts of nerve growth factor (NGF) in the control and transgenic pineal glands, suggesting that it occurred in a NGF- independent manner. The results suggest that target neurotransmitter phenotype influences the formation of afferent connections during development.

AB - Neurotransmitters play a variety of important roles during nervous system development. In the present study, we hypothesized that neurotransmitter phenotype of both projecting and target cells is an important factor for the final synaptic linkage and its specificity. To test this hypothesis, we used transgenic techniques to convert serotonin/melatonin-producing cells of the pineal gland into cells that also produce dopamine and investigated the innervation of the phenotypically altered target cells. This phenotypic alteration markedly reduced the noradrenergic innervation originating from the superior cervical ganglia. Although the mechanism by which the reduction occurs is presently unknown, quantitative enzyme-linked immunoassay showed the presence of the equivalent amounts of nerve growth factor (NGF) in the control and transgenic pineal glands, suggesting that it occurred in a NGF- independent manner. The results suggest that target neurotransmitter phenotype influences the formation of afferent connections during development.

KW - dopamine

KW - pineal gland

KW - superior cervical ganglion

KW - tyrosine hydroxylase

UR - http://www.scopus.com/inward/record.url?scp=0029920990&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029920990&partnerID=8YFLogxK

U2 - 10.1073/pnas.93.7.2862

DO - 10.1073/pnas.93.7.2862

M3 - Article

C2 - 8610132

AN - SCOPUS:0029920990

VL - 93

SP - 2862

EP - 2866

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 7

ER -