Recombinant lectin microarrays for glycomic analysis.

Daniel C. Propheter, Ku Lung Hsu, Lara K. Mahal

Research output: Contribution to journalArticle

Abstract

The cell surface is covered with a myriad of carbohydrates that form a complex matrix of oligosaccharides. Carbohydrate recognition plays critical roles in pathogenesis, trafficking, and differentiation. Lectin microarray technology presents a novel platform for the high-throughput analysis of these structurally diverse biopolymers. One drawback of this technology has been limitations imposed by the commercially available plant lectins used in the array. Not only are a majority of these plant-derived proteins glycosylated, which can complicate glycomic analysis, but they also differ in activity and availability. Our lab has recently introduced recombinant lectins to enhance the stability and scope of our lectin panel. Herein, we provide a detailed procedure for the expression of bacterially-derived lectins and their application to a recombinant lectin microarray.

Original languageEnglish (US)
Pages (from-to)67-77
Number of pages11
JournalMethods in Molecular Biology
Volume723
DOIs
StatePublished - 2011

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Glycomics
Microarray Analysis
Lectins
Carbohydrates
Plant Lectins
Technology
Plant Proteins
Biopolymers
Oligosaccharides

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Recombinant lectin microarrays for glycomic analysis. / Propheter, Daniel C.; Hsu, Ku Lung; Mahal, Lara K.

In: Methods in Molecular Biology, Vol. 723, 2011, p. 67-77.

Research output: Contribution to journalArticle

Propheter, Daniel C. ; Hsu, Ku Lung ; Mahal, Lara K. / Recombinant lectin microarrays for glycomic analysis. In: Methods in Molecular Biology. 2011 ; Vol. 723. pp. 67-77.
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