Recapturing and trapping single molecules with a solid-state nanopore

Marc Gershow, J. A. Golovchenko

    Research output: Contribution to journalArticle

    Abstract

    The development of solid-state nanopores, inspired by their biological counterparts, shows great potential for the study of single macromolecules. Applications such as DNA sequencing and the exploration of protein folding require control of the dynamics of the molecule's interaction with the pore, but DNA capture by a solid-state nanopore is not well understood. By recapturing individual molecules soon after they pass through a nanopore, we reveal the mechanism by which double-stranded DNA enters the pore. The observed recapture rates and times agree with solutions of a drift-diffusion model. Electric forces draw DNA to the pore over micrometer-scale distances, and upon arrival at the pore, molecules begin translocation almost immediately. Repeated translocation of the same molecule improves measurement accuracy, offers a way to probe the chemical transformations and internal dynamics of macromolecules on sub-millisecond time and sub-micrometre length scales, and demonstrates the ability to trap, study and manipulate individual macromolecules in solution.

    Original languageEnglish (US)
    Pages (from-to)775-779
    Number of pages5
    JournalNature Nanotechnology
    Volume2
    Issue number12
    DOIs
    StatePublished - Dec 2007

    Fingerprint

    Nanopores
    DNA
    deoxyribonucleic acid
    Macromolecules
    trapping
    macromolecules
    solid state
    porosity
    Molecules
    micrometers
    molecules
    Protein folding
    sequencing
    folding
    arrivals
    traps
    proteins
    probes
    interactions

    ASJC Scopus subject areas

    • Bioengineering
    • Biomedical Engineering
    • Electrical and Electronic Engineering
    • Materials Science(all)
    • Condensed Matter Physics
    • Atomic and Molecular Physics, and Optics

    Cite this

    Recapturing and trapping single molecules with a solid-state nanopore. / Gershow, Marc; Golovchenko, J. A.

    In: Nature Nanotechnology, Vol. 2, No. 12, 12.2007, p. 775-779.

    Research output: Contribution to journalArticle

    Gershow, Marc ; Golovchenko, J. A. / Recapturing and trapping single molecules with a solid-state nanopore. In: Nature Nanotechnology. 2007 ; Vol. 2, No. 12. pp. 775-779.
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