Real-time PCR improves detection of Trichomonas vaginalis infection compared with culture using self-collected vaginal swabs

Angela M. Caliendo, J. A. Jordan, A. M. Green, J. Ingersoll, Ralph DiClemente, G. M. Wingood

Research output: Contribution to journalArticle

Abstract

Objective. To compare a real-time polymerase chain reaction (PCR) assay with broth culture for the detection of Trichomonas vaginalis using self-collected vaginal swabs. Methods. Self-collected vaginal swabs were obtained from adolescent and young adult African-American women participating in HIV-1 prevention programs. T. vaginalis culture was performed using the InPouch TV System. Samples for the real-time PCR assay were collected using the BDProbeTec ET Culturette Direct Dry Swab system and tested in a laboratory-developed assay which targeted a repeated sequence of the genome. Discrepant samples that were culture negative and positive in the real-time PCR assay were tested in a confirmatory PCR which targeted a different region of the T. vaginalis genome, the 18S ribosomal DNA gene. Results. Of the 524 specimens tested by both culture and real-time PCR, 36 were culture positive and 54 were positive in the real-time PCR assay; 16 of the 18 discrepant specimens were also positive in the confirmatory PCR assay. Using a modified gold standard of positive by culture or positive in both PCR assays, the sensitivity of the real-time PCR assay was 100% and the specificity was 99.6%, whereas culture had a sensitivity of 69.2% and a specificity of 100%. Conclusions. The real-time PCR assay was sensitive and specific for the detection of T. vaginalis DNA from self-collected vaginal swab specimens. The ability to use the BDProbeTec dry swab system for the real-time PCR testing allowed for the detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and T. vaginalis from a single specimen.

Original languageEnglish (US)
Pages (from-to)145-150
Number of pages6
JournalInfectious Diseases in Obstetrics and Gynecology
Volume13
Issue number3
DOIs
StatePublished - Sep 1 2005

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Trichomonas Infections
Trichomonas vaginalis
Real-Time Polymerase Chain Reaction
Polymerase Chain Reaction
Genome
Neisseria gonorrhoeae
Chlamydia trachomatis
Ribosomal DNA
African Americans
HIV-1
Young Adult

Keywords

  • Adolescent women
  • DNA
  • Trichomoniasis

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Dermatology

Cite this

Real-time PCR improves detection of Trichomonas vaginalis infection compared with culture using self-collected vaginal swabs. / Caliendo, Angela M.; Jordan, J. A.; Green, A. M.; Ingersoll, J.; DiClemente, Ralph; Wingood, G. M.

In: Infectious Diseases in Obstetrics and Gynecology, Vol. 13, No. 3, 01.09.2005, p. 145-150.

Research output: Contribution to journalArticle

Caliendo, Angela M. ; Jordan, J. A. ; Green, A. M. ; Ingersoll, J. ; DiClemente, Ralph ; Wingood, G. M. / Real-time PCR improves detection of Trichomonas vaginalis infection compared with culture using self-collected vaginal swabs. In: Infectious Diseases in Obstetrics and Gynecology. 2005 ; Vol. 13, No. 3. pp. 145-150.
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abstract = "Objective. To compare a real-time polymerase chain reaction (PCR) assay with broth culture for the detection of Trichomonas vaginalis using self-collected vaginal swabs. Methods. Self-collected vaginal swabs were obtained from adolescent and young adult African-American women participating in HIV-1 prevention programs. T. vaginalis culture was performed using the InPouch TV System. Samples for the real-time PCR assay were collected using the BDProbeTec ET Culturette Direct Dry Swab system and tested in a laboratory-developed assay which targeted a repeated sequence of the genome. Discrepant samples that were culture negative and positive in the real-time PCR assay were tested in a confirmatory PCR which targeted a different region of the T. vaginalis genome, the 18S ribosomal DNA gene. Results. Of the 524 specimens tested by both culture and real-time PCR, 36 were culture positive and 54 were positive in the real-time PCR assay; 16 of the 18 discrepant specimens were also positive in the confirmatory PCR assay. Using a modified gold standard of positive by culture or positive in both PCR assays, the sensitivity of the real-time PCR assay was 100{\%} and the specificity was 99.6{\%}, whereas culture had a sensitivity of 69.2{\%} and a specificity of 100{\%}. Conclusions. The real-time PCR assay was sensitive and specific for the detection of T. vaginalis DNA from self-collected vaginal swab specimens. The ability to use the BDProbeTec dry swab system for the real-time PCR testing allowed for the detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and T. vaginalis from a single specimen.",
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AB - Objective. To compare a real-time polymerase chain reaction (PCR) assay with broth culture for the detection of Trichomonas vaginalis using self-collected vaginal swabs. Methods. Self-collected vaginal swabs were obtained from adolescent and young adult African-American women participating in HIV-1 prevention programs. T. vaginalis culture was performed using the InPouch TV System. Samples for the real-time PCR assay were collected using the BDProbeTec ET Culturette Direct Dry Swab system and tested in a laboratory-developed assay which targeted a repeated sequence of the genome. Discrepant samples that were culture negative and positive in the real-time PCR assay were tested in a confirmatory PCR which targeted a different region of the T. vaginalis genome, the 18S ribosomal DNA gene. Results. Of the 524 specimens tested by both culture and real-time PCR, 36 were culture positive and 54 were positive in the real-time PCR assay; 16 of the 18 discrepant specimens were also positive in the confirmatory PCR assay. Using a modified gold standard of positive by culture or positive in both PCR assays, the sensitivity of the real-time PCR assay was 100% and the specificity was 99.6%, whereas culture had a sensitivity of 69.2% and a specificity of 100%. Conclusions. The real-time PCR assay was sensitive and specific for the detection of T. vaginalis DNA from self-collected vaginal swab specimens. The ability to use the BDProbeTec dry swab system for the real-time PCR testing allowed for the detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and T. vaginalis from a single specimen.

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