Real-time measurement of anchorage-dependent cell adhesion using a Quartz Crystal Microbalance

David M. Gryte, Michael Ward, Wei Shou Hu

Research output: Contribution to journalArticle

Abstract

An in situ technique based on the mass-sensitive piezoelectric quartz crystal microbalance (QCM) was applied to continuously monitor the attachment and detachment of anchorage-dependent mammalian cells on a metal surface. Specifically, we have demonstrated that the attachment of Vero cells to metal surfaces on the piezoelectrically active area of the QCM results in decreases in the QCM resonant frequency that can be monitored conveniently in real time. Lysis and detachment of Vero cells caused by vesicular stomatitis virus (VSV) infection can also be monitored readily. Our results demonstrate that the QCM is a viable technique for monitoring anchorage-dependent cell attachment and detachment on surfaces (caused by stimulatory, inhibitory, or cytopathic effects).

Original languageEnglish (US)
Pages (from-to)105-108
Number of pages4
JournalBiotechnology Progress
Volume9
Issue number1
StatePublished - 1993

Fingerprint

Quartz Crystal Microbalance Techniques
quartz
cell adhesion
Cell Adhesion
crystals
Vero Cells
Metals
metals
cells
Vesiculovirus
Vesicular Stomatitis
cytopathogenicity
monitoring
Virus Diseases
methodology
infection

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Microbiology

Cite this

Real-time measurement of anchorage-dependent cell adhesion using a Quartz Crystal Microbalance. / Gryte, David M.; Ward, Michael; Hu, Wei Shou.

In: Biotechnology Progress, Vol. 9, No. 1, 1993, p. 105-108.

Research output: Contribution to journalArticle

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