Reactivation of horseradish peroxidase in organic solvent using extraction

Jin Kim, Yoo Seong Choi, Young Je Yoo

Research output: Contribution to journalArticle

Abstract

A novel extraction method was developed aiming at increasing the stability of enzymes in organic solvent media. Horseradish peroxidase (HRP), inactivated in a tetrahydrofuran (THF)/water (1:1, v/v), regained and maintained its activity when HRP was extracted by adding a THF/benzene mixture to the original solution. However, the HRP activity was drastically lowered in the enzyme-free blank solution that had been formed by employing the same extraction procedure. As a result, the reactivation after the extraction is believed to depend on enzyme history, and might be arisen from an irreversible structural change of the enzyme.

Original languageEnglish (US)
Pages (from-to)485-489
Number of pages5
JournalBiotechnology Letters
Volume22
Issue number6
DOIs
StatePublished - 2000

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Solvent extraction
Horseradish Peroxidase
Organic solvents
Enzymes
Enzyme Stability
Benzene
History
Water
tetrahydrofuran

Keywords

  • Enzyme
  • Extraction
  • Horseradish peroxidase
  • Organic solvent
  • Reactivation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Reactivation of horseradish peroxidase in organic solvent using extraction. / Kim, Jin; Choi, Yoo Seong; Yoo, Young Je.

In: Biotechnology Letters, Vol. 22, No. 6, 2000, p. 485-489.

Research output: Contribution to journalArticle

Kim, Jin ; Choi, Yoo Seong ; Yoo, Young Je. / Reactivation of horseradish peroxidase in organic solvent using extraction. In: Biotechnology Letters. 2000 ; Vol. 22, No. 6. pp. 485-489.
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