Ratiometric lectin microarray analysis of the mammalian cell surface glycome.

Ku Lung Hsu, Kanoelani Pilobello, Lakshmipriya Krishnamoorthy, Lara K. Mahal

Research output: Contribution to journalArticle

Abstract

The mammalian cell surface is rich with carbohydrate polymers involved in a diversity of biological recognition events. Dynamic alterations of surface glycans mediate cell-cell communication in the immune system and host specificity of bacterial and viral pathogens. In addition, altered surface glycosylation has been implicated in disease progression of many cancers and may serve as important new targets for therapeutics. Despite the importance of glycosylation, the systematic analysis of sugars, i.e., glycomics, has lagged behind the well-studied disciplines of genomics and proteomics. This deficiency is due in part to the unique analytical challenges presented by glycans and the overwhelming diversity of sugars in nature. New microarray technologies have provided a high-throughput methods with which to probe the glycome. Our laboratory has pioneered a shown ratiometric two-color lectin microarray method that rapidly evaluates differences in the glycosylation of mammalian cells. Herein, we present a detailed protocol of our lectin microarray methodology for the differential analysis of mammalian glycomes.

Original languageEnglish (US)
Pages (from-to)117-131
Number of pages15
JournalMethods in Molecular Biology
Volume671
StatePublished - 2011

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Tissue Array Analysis
Glycosylation
Lectins
Polysaccharides
Glycomics
Host Specificity
Biodiversity
Genomics
Cell Communication
Proteomics
Disease Progression
Immune System
Polymers
Color
Carbohydrates
Technology
Neoplasms
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

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Ratiometric lectin microarray analysis of the mammalian cell surface glycome. / Hsu, Ku Lung; Pilobello, Kanoelani; Krishnamoorthy, Lakshmipriya; Mahal, Lara K.

In: Methods in Molecular Biology, Vol. 671, 2011, p. 117-131.

Research output: Contribution to journalArticle

Hsu, Ku Lung ; Pilobello, Kanoelani ; Krishnamoorthy, Lakshmipriya ; Mahal, Lara K. / Ratiometric lectin microarray analysis of the mammalian cell surface glycome. In: Methods in Molecular Biology. 2011 ; Vol. 671. pp. 117-131.
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