Rapid increase of calcitonin-specific mRNA after acute hypercalcemia.

N. Segond, A. Jullienne, F. Lasmoles, C. Desplan, G. Milhaud, M. S. Moukhtar

Research output: Contribution to journalArticle

Abstract

The regulation of calcitonin (CT) secretion by calcium was studied by measuring CT mRNA extracted from thyroids of normal rats subjected to acute calcium stimulation in vivo. The 15000-Mr primary translation product of CT mRNA was identified by immunoprecipitation using specific antibodies. While total mRNA and total radioactivity incorporated after translation of total mRNA remained unaffected by the calcium stimulation, a fourfold increase in radioactivity incorporated in CT primary translation product occurred as early as 2 min after calcium administration. This peak coincided with a rise in plasma levels of the hormone and preceded a detectable decrease in tissue stores. These results suggest that calcium ion, either directly or indirectly via its action on intracellular stores of the hormone or its precursors, causes a rapid increase in cell levels of translatable CT mRNA. In view of the extremely short time (2 min) in which this increase occurs, the action is probably at the post-transcriptional level as no increase in CT mRNA levels could be detected by hybridization assay using a specific cDNA probe for human CT mRNA.

Original languageEnglish (US)
Pages (from-to)209-215
Number of pages7
JournalEuropean Journal of Biochemistry
Volume139
Issue number2
StatePublished - Mar 1 1984

Fingerprint

Calcitonin
Hypercalcemia
Messenger RNA
Calcium
Radioactivity
Hormones
Protein Biosynthesis
Immunoprecipitation
Rats
Assays
Thyroid Gland
Complementary DNA
Ions
Tissue
Plasmas
Antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Segond, N., Jullienne, A., Lasmoles, F., Desplan, C., Milhaud, G., & Moukhtar, M. S. (1984). Rapid increase of calcitonin-specific mRNA after acute hypercalcemia. European Journal of Biochemistry, 139(2), 209-215.

Rapid increase of calcitonin-specific mRNA after acute hypercalcemia. / Segond, N.; Jullienne, A.; Lasmoles, F.; Desplan, C.; Milhaud, G.; Moukhtar, M. S.

In: European Journal of Biochemistry, Vol. 139, No. 2, 01.03.1984, p. 209-215.

Research output: Contribution to journalArticle

Segond, N, Jullienne, A, Lasmoles, F, Desplan, C, Milhaud, G & Moukhtar, MS 1984, 'Rapid increase of calcitonin-specific mRNA after acute hypercalcemia.', European Journal of Biochemistry, vol. 139, no. 2, pp. 209-215.
Segond N, Jullienne A, Lasmoles F, Desplan C, Milhaud G, Moukhtar MS. Rapid increase of calcitonin-specific mRNA after acute hypercalcemia. European Journal of Biochemistry. 1984 Mar 1;139(2):209-215.
Segond, N. ; Jullienne, A. ; Lasmoles, F. ; Desplan, C. ; Milhaud, G. ; Moukhtar, M. S. / Rapid increase of calcitonin-specific mRNA after acute hypercalcemia. In: European Journal of Biochemistry. 1984 ; Vol. 139, No. 2. pp. 209-215.
@article{d32449cde8ac4367a2a8080a9c10de48,
title = "Rapid increase of calcitonin-specific mRNA after acute hypercalcemia.",
abstract = "The regulation of calcitonin (CT) secretion by calcium was studied by measuring CT mRNA extracted from thyroids of normal rats subjected to acute calcium stimulation in vivo. The 15000-Mr primary translation product of CT mRNA was identified by immunoprecipitation using specific antibodies. While total mRNA and total radioactivity incorporated after translation of total mRNA remained unaffected by the calcium stimulation, a fourfold increase in radioactivity incorporated in CT primary translation product occurred as early as 2 min after calcium administration. This peak coincided with a rise in plasma levels of the hormone and preceded a detectable decrease in tissue stores. These results suggest that calcium ion, either directly or indirectly via its action on intracellular stores of the hormone or its precursors, causes a rapid increase in cell levels of translatable CT mRNA. In view of the extremely short time (2 min) in which this increase occurs, the action is probably at the post-transcriptional level as no increase in CT mRNA levels could be detected by hybridization assay using a specific cDNA probe for human CT mRNA.",
author = "N. Segond and A. Jullienne and F. Lasmoles and C. Desplan and G. Milhaud and Moukhtar, {M. S.}",
year = "1984",
month = "3",
day = "1",
language = "English (US)",
volume = "139",
pages = "209--215",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Rapid increase of calcitonin-specific mRNA after acute hypercalcemia.

AU - Segond, N.

AU - Jullienne, A.

AU - Lasmoles, F.

AU - Desplan, C.

AU - Milhaud, G.

AU - Moukhtar, M. S.

PY - 1984/3/1

Y1 - 1984/3/1

N2 - The regulation of calcitonin (CT) secretion by calcium was studied by measuring CT mRNA extracted from thyroids of normal rats subjected to acute calcium stimulation in vivo. The 15000-Mr primary translation product of CT mRNA was identified by immunoprecipitation using specific antibodies. While total mRNA and total radioactivity incorporated after translation of total mRNA remained unaffected by the calcium stimulation, a fourfold increase in radioactivity incorporated in CT primary translation product occurred as early as 2 min after calcium administration. This peak coincided with a rise in plasma levels of the hormone and preceded a detectable decrease in tissue stores. These results suggest that calcium ion, either directly or indirectly via its action on intracellular stores of the hormone or its precursors, causes a rapid increase in cell levels of translatable CT mRNA. In view of the extremely short time (2 min) in which this increase occurs, the action is probably at the post-transcriptional level as no increase in CT mRNA levels could be detected by hybridization assay using a specific cDNA probe for human CT mRNA.

AB - The regulation of calcitonin (CT) secretion by calcium was studied by measuring CT mRNA extracted from thyroids of normal rats subjected to acute calcium stimulation in vivo. The 15000-Mr primary translation product of CT mRNA was identified by immunoprecipitation using specific antibodies. While total mRNA and total radioactivity incorporated after translation of total mRNA remained unaffected by the calcium stimulation, a fourfold increase in radioactivity incorporated in CT primary translation product occurred as early as 2 min after calcium administration. This peak coincided with a rise in plasma levels of the hormone and preceded a detectable decrease in tissue stores. These results suggest that calcium ion, either directly or indirectly via its action on intracellular stores of the hormone or its precursors, causes a rapid increase in cell levels of translatable CT mRNA. In view of the extremely short time (2 min) in which this increase occurs, the action is probably at the post-transcriptional level as no increase in CT mRNA levels could be detected by hybridization assay using a specific cDNA probe for human CT mRNA.

UR - http://www.scopus.com/inward/record.url?scp=0021402149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021402149&partnerID=8YFLogxK

M3 - Article

VL - 139

SP - 209

EP - 215

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 2

ER -