Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Talita Castro, Maite Sabalza, Cheryl Barber, William Abrams, Antonio Charlys Da Costa, Flavio Augusto De Pádua Milagres, Paulo Henrique Braz-Silva, Daniel Malamud, Marina Gallottini

Research output: Contribution to journalArticle

Abstract

Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2%) were positive for ZIKV with an average time to positivity (Tp) of 13.5 min, and from the 63 urine samples, 25 (39.7%) were positive with the average Tp of 15.8 min. Saliva detected more samples (p = 0.0042) and had faster Tp (p = 0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP.

Original languageEnglish (US)
Article number1510712
JournalJournal of Oral Microbiology
Volume10
Issue number1
DOIs
StatePublished - Jan 1 2018

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Saliva
Urine
Flaviviridae
RNA
RNA Viruses
RNA-Directed DNA Polymerase
Zika Virus
Nucleic Acids
Real-Time Polymerase Chain Reaction
Zika Virus Infection

Keywords

  • Dengue
  • Flavivirus
  • infection
  • LAMP
  • polymerase chain reaction
  • RNA
  • saliva
  • virology
  • Zika

ASJC Scopus subject areas

  • Dentistry (miscellaneous)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Castro, T., Sabalza, M., Barber, C., Abrams, W., Da Costa, A. C., De Pádua Milagres, F. A., ... Gallottini, M. (2018). Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP). Journal of Oral Microbiology, 10(1), [1510712]. https://doi.org/10.1080/20002297.2018.1510712

Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP). / Castro, Talita; Sabalza, Maite; Barber, Cheryl; Abrams, William; Da Costa, Antonio Charlys; De Pádua Milagres, Flavio Augusto; Braz-Silva, Paulo Henrique; Malamud, Daniel; Gallottini, Marina.

In: Journal of Oral Microbiology, Vol. 10, No. 1, 1510712, 01.01.2018.

Research output: Contribution to journalArticle

Castro, T, Sabalza, M, Barber, C, Abrams, W, Da Costa, AC, De Pádua Milagres, FA, Braz-Silva, PH, Malamud, D & Gallottini, M 2018, 'Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)', Journal of Oral Microbiology, vol. 10, no. 1, 1510712. https://doi.org/10.1080/20002297.2018.1510712
Castro, Talita ; Sabalza, Maite ; Barber, Cheryl ; Abrams, William ; Da Costa, Antonio Charlys ; De Pádua Milagres, Flavio Augusto ; Braz-Silva, Paulo Henrique ; Malamud, Daniel ; Gallottini, Marina. / Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP). In: Journal of Oral Microbiology. 2018 ; Vol. 10, No. 1.
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abstract = "Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2{\%}) were positive for ZIKV with an average time to positivity (Tp) of 13.5 min, and from the 63 urine samples, 25 (39.7{\%}) were positive with the average Tp of 15.8 min. Saliva detected more samples (p = 0.0042) and had faster Tp (p = 0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP.",
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AU - De Pádua Milagres, Flavio Augusto

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