Rapid detection of exosomal microRNA biomarkers by electrokinetic concentration for liquid biopsy on chip

Lucia S. Cheung, Xi Wei, Diogo Martins, Rafael Song

Research output: Contribution to journalArticle

Abstract

An ion concentration polarization (ICP)-based electrokinetic concentration device is used for accelerating the surface hybridization reaction between exosomal microRNAs (miRNAs) and morpholinos (MOs) as a synthetic oligo capture probe in the nanomolar concentration range in a microfluidic channel. Compared with standard hybridization at the same concentration, the hybridization time of the miRNA target on MO capture probes could be reduced from ∼24 h to 30 min, with an increase in detection speed by 48 times. This ICP-enhanced hybridization method not only significantly decreases the detection time but also makes workflow simple to use, circumventing use of quantitative reverse transcription polymerase chain reaction or other conventional enzyme-based amplification methods that can cause artifacts.

Original languageEnglish (US)
Article number014104
JournalBiomicrofluidics
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2018

Fingerprint

Morpholinos
Biopsy
biomarkers
electrokinetics
Biomarkers
MicroRNAs
chips
Ions
Polarization
ion concentration
Microfluidics
Workflow
Polymerase chain reaction
Surface reactions
Liquids
Transcription
liquids
Artifacts
Reverse Transcription
Amplification

ASJC Scopus subject areas

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Cite this

Rapid detection of exosomal microRNA biomarkers by electrokinetic concentration for liquid biopsy on chip. / Cheung, Lucia S.; Wei, Xi; Martins, Diogo; Song, Rafael.

In: Biomicrofluidics, Vol. 12, No. 1, 014104, 01.01.2018.

Research output: Contribution to journalArticle

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