Rapid and potent induction of cell death and loss of NK cell cytotoxicity against oral tumors by F(ab′)2 fragment of anti-CD16 antibody

Anahid Jewett, Antonia Teruel, Marcela Romero-Reyes, Christian Head, Nicholas Cacalano

Research output: Contribution to journalArticle

Abstract

Freshly isolated untreated NK cells undergo rapid apoptosis and lose their cytotoxic function upon the addition of F(ab′)2 fragment of anti-CD16 antibodies. Loss of NK cell cytotoxic function after treatment with F(ab′)2 fragment of anti-CD16 antibody can be seen against K562 and UCLA-2 oral tumor cells when either added immediately in the co-cultures of NK cells with the tumor cells or after pre-treatment of NK cells with the antibody before their addition to the tumor cells. Addition of Interleukin-2 (IL-2) in combination with anti-CD16 antibody to NK cells delayed the induction of DNA fragmentation in NK cells, and even though decreased cytotoxicity could still be observed against K562 and UCLA-2 oral tumors when compared to IL-2 alone treated NK cells, the cytotoxicity levels remained relatively higher and approached those obtained by untreated NK cells in the absence of antibody treatment. No increases in IFN-γ, Granzymes A and B, Perforin and TRAIL genes could be seen in NK cells treated with anti-CD16 antibody. Neither secretion of IFN-γ nor increased expression of CD69 activation antigen could be observed after the treatment of NK cells with anti-CD16 antibody. Furthermore, IL-2 mediated increase in CD69 surface antigens was down-modulated by anti-CD16 antibody. Finally, the addition of anti-CD16 antibody to co-cultures of NK cells with tumor target cells was not inhibitory for the secretion of VEGF by oral tumor cells, unlike those co-cultured with untreated or IL-2 treated NK cells. Thus, binding and triggering of CD16 receptor on NK cells may enhance oral tumor survival and growth by decreased ability of NK cells to suppress VEGF secretion or induce tumor cell death during the interaction of NK cells with oral tumor cells.

Original languageEnglish (US)
Pages (from-to)1053-1066
Number of pages14
JournalCancer Immunology, Immunotherapy
Volume57
Issue number7
DOIs
StatePublished - Jul 2008

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Natural Killer Cells
Anti-Idiotypic Antibodies
Cell Death
Neoplasms
Interleukin-2
Granzymes
Coculture Techniques
Vascular Endothelial Growth Factor A
Natural Killer Cell Receptors
Perforin
Antibodies
DNA Fragmentation
Surface Antigens
Apoptosis

Keywords

  • Apoptosis
  • CD16
  • Natural killer cells
  • Oral tumors
  • VEGF

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • Oncology

Cite this

Rapid and potent induction of cell death and loss of NK cell cytotoxicity against oral tumors by F(ab′)2 fragment of anti-CD16 antibody. / Jewett, Anahid; Teruel, Antonia; Romero-Reyes, Marcela; Head, Christian; Cacalano, Nicholas.

In: Cancer Immunology, Immunotherapy, Vol. 57, No. 7, 07.2008, p. 1053-1066.

Research output: Contribution to journalArticle

Jewett, Anahid ; Teruel, Antonia ; Romero-Reyes, Marcela ; Head, Christian ; Cacalano, Nicholas. / Rapid and potent induction of cell death and loss of NK cell cytotoxicity against oral tumors by F(ab′)2 fragment of anti-CD16 antibody. In: Cancer Immunology, Immunotherapy. 2008 ; Vol. 57, No. 7. pp. 1053-1066.
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