Quasispecies of bovine enteric and respiratory coronaviruses based on complete genome sequences and genetic changes after tissue culture adaptation

Xinsheng Zhang, Mustafa Hasoksuz, David Spiro, Rebecca Halpin, Shiliang Wang, Anastasia Vlasova, Daniel Janies, Leandro R. Jones, Elodie Ghedin, Linda J. Saif

Research output: Contribution to journalArticle

Abstract

The genetic diversity of 2 pairs (AH65 and AH187) of wild type bovine coronaviruses (BCoV) sequenced directly from nasal (respiratory) and rectal (enteric) swabs of two feedlot calves with respiratory and enteric symptoms [Hasoksuz, M., Sreevatsan, S., Cho, K.O., Hoet, A.E., Saif, L.J., 2002b. Molecular analysis of the S1 subunit of the spike glycoprotein of respiratory and enteric bovine coronavirus isolates. Virus Res. 84 (1-2), 101-109.]. was analyzed. Sequence analysis of the complete genomes revealed differences at 123 and 149 nucleotides (nt) throughout the entire genome between the respiratory and enteric strains for samples AH65 and AH187, respectively, indicating the presence of intra-host BCoV quasispecies. In addition, significant numbers of sequence ambiguities were found in the genomes of some BCoV-R and BCoV-E strains, suggesting intra-isolate quasispecies. The tissue culture (TC) passaged counterparts of AH65 respiratory BCoV (AH65-R-TC) and enteric BCoV (AH65-E-TC) were also sequenced after 14 and 15 passages and 1 plaque purification in human rectal tumor cells (HRT-18), respectively. Compared to the parental wild type strains, tissue culture passage generated 104 nt changes in the AH65-E-TC isolate but only 8 nt changes in the AH65-R-TC isolate. Particularly noteworthy, the majority of nucleotide changes in the AH65-E-TC isolate occurred at the identical positions as the mutations occurring in the AH65-R strain from the same animal. These data suggest that BCoV evolves through quasispecies development, and that enteric BCoV isolates are more prone to genetic changes and may mutate to resemble respiratory BCoV strains after tissue culture passage.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalVirology
Volume363
Issue number1
DOIs
StatePublished - Jun 20 2007

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Bovine Coronavirus
Coronavirus
Genome
Nucleotides
Rectal Neoplasms
Nose
Sequence Analysis
Glycoproteins

Keywords

  • Complete genomes
  • Enteric and respiratory bovine coronaviruses
  • Mutations
  • Quasispecies
  • Tissue and culture adaptation and passage

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Quasispecies of bovine enteric and respiratory coronaviruses based on complete genome sequences and genetic changes after tissue culture adaptation. / Zhang, Xinsheng; Hasoksuz, Mustafa; Spiro, David; Halpin, Rebecca; Wang, Shiliang; Vlasova, Anastasia; Janies, Daniel; Jones, Leandro R.; Ghedin, Elodie; Saif, Linda J.

In: Virology, Vol. 363, No. 1, 20.06.2007, p. 1-10.

Research output: Contribution to journalArticle

Zhang, Xinsheng ; Hasoksuz, Mustafa ; Spiro, David ; Halpin, Rebecca ; Wang, Shiliang ; Vlasova, Anastasia ; Janies, Daniel ; Jones, Leandro R. ; Ghedin, Elodie ; Saif, Linda J. / Quasispecies of bovine enteric and respiratory coronaviruses based on complete genome sequences and genetic changes after tissue culture adaptation. In: Virology. 2007 ; Vol. 363, No. 1. pp. 1-10.
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