Quantitative regulation of the dynamic steady state of actin networks

Angelika Manhart, Téa Aleksandra Icheva, Christophe Guerin, Tobbias Klar, Rajaa Boujemaa-Paterski, Manuel Thery, Laurent Blanchoin, Alexander Mogilner

Research output: Contribution to journalArticle

Abstract

Principles of regulation of actin network dimensions are fundamentally important for cell functions, yet remain unclear. Using both in vitro and in silico approaches, we studied the effect of key parameters, such as actin density, ADF/Cofilin concentration and network width on the network length. In the presence of ADF/Cofilin, networks reached equilibrium and became treadmilling. At the trailing edge, the network disintegrated into large fragments. A mathematical model predicts the network length as a function of width, actin and ADF/Cofilin concentrations. Local depletion of ADF/Cofilin by binding to actin is significant, leading to wider networks growing longer. A single rate of breaking network nodes, proportional to ADF/Cofilin density and inversely proportional to the square of the actin density, can account for the disassembly dynamics. Selective disassembly of heterogeneous networks by ADF/Cofilin controls steering during motility. Our results establish general principles on how the dynamic steady state of actin network emerges from biochemical and structural feedbacks.

Original languageEnglish (US)
JournaleLife
Volume8
DOIs
StatePublished - Mar 14 2019

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Actin Depolymerizing Factors
Actins
Physiological Feedback
Heterogeneous networks
Computer Simulation
Theoretical Models
Mathematical models
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Keywords

  • actin
  • actin disassembly
  • actin network length
  • ADF/Cofilin
  • cell biology
  • none

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Manhart, A., Icheva, T. A., Guerin, C., Klar, T., Boujemaa-Paterski, R., Thery, M., ... Mogilner, A. (2019). Quantitative regulation of the dynamic steady state of actin networks. eLife, 8. https://doi.org/10.7554/eLife.42413

Quantitative regulation of the dynamic steady state of actin networks. / Manhart, Angelika; Icheva, Téa Aleksandra; Guerin, Christophe; Klar, Tobbias; Boujemaa-Paterski, Rajaa; Thery, Manuel; Blanchoin, Laurent; Mogilner, Alexander.

In: eLife, Vol. 8, 14.03.2019.

Research output: Contribution to journalArticle

Manhart, A, Icheva, TA, Guerin, C, Klar, T, Boujemaa-Paterski, R, Thery, M, Blanchoin, L & Mogilner, A 2019, 'Quantitative regulation of the dynamic steady state of actin networks', eLife, vol. 8. https://doi.org/10.7554/eLife.42413
Manhart A, Icheva TA, Guerin C, Klar T, Boujemaa-Paterski R, Thery M et al. Quantitative regulation of the dynamic steady state of actin networks. eLife. 2019 Mar 14;8. https://doi.org/10.7554/eLife.42413
Manhart, Angelika ; Icheva, Téa Aleksandra ; Guerin, Christophe ; Klar, Tobbias ; Boujemaa-Paterski, Rajaa ; Thery, Manuel ; Blanchoin, Laurent ; Mogilner, Alexander. / Quantitative regulation of the dynamic steady state of actin networks. In: eLife. 2019 ; Vol. 8.
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