Quantitative analysis of germline mitosis in adult C. elegans

John Maciejowski, Nadia Ugel, Bhubaneswar Mishra, Marco Isopi, E. Jane Albert Hubbard

Research output: Contribution to journalArticle

Abstract

Certain aspects of the distal gonad of C. elegans are comparable to niche/stem cell systems in other organisms. The distal tip cell (DTC) caps a blind-ended tube; only the distal germ cells maintain proliferation in response to signaling from the DTC via the GLP-1/Notch signaling pathway in the germ line. Fruitful comparison between this system and other stem cell systems is limited by a lack of basic information regarding germ cell division behavior in C. elegans. Here, we explore the spatial pattern of cell division frequency in the adult C. elegans germ line relative to distance from the distal tip. We mapped the positions of actively dividing germline nuclei in over 600 fixed gonad preparations including the wild type and a gain-of-function ligand-responsive GLP-1 receptor mutant with an extended mitotic zone. One particularly surprising observation from these data is that the frequency of cell divisions is lower in distal-most cells-cells that directly contact the distal tip cell body-relative to cells further proximal, a difference that persists in the gain-of-function GLP-1 mutant. These results suggest that cell division frequency in the distal-most cells may be suppressed or otherwise controlled in a complex manner. Further, our data suggest that the presence of an active cell division influences the probability of observing simultaneous cell divisions in the same gonad arm, and that simultaneous divisions tend to cluster spatially. We speculate that this system behaves similarly to niche/stem cell/transit amplifying cell systems in other organisms.

Original languageEnglish (US)
Pages (from-to)142-151
Number of pages10
JournalDevelopmental Biology
Volume292
Issue number1
DOIs
StatePublished - Apr 1 2006

Fingerprint

Mitosis
Cell Division
Germ Cells
Gonads
Stem Cell Niche
Glucagon-Like Peptide 1
Stem Cells
Cell Proliferation
Observation
Ligands

Keywords

  • C. elegans
  • Germ line
  • GLP-1
  • Intensity profile
  • Mitosis
  • Spatial statistics
  • Stem cell

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Quantitative analysis of germline mitosis in adult C. elegans. / Maciejowski, John; Ugel, Nadia; Mishra, Bhubaneswar; Isopi, Marco; Hubbard, E. Jane Albert.

In: Developmental Biology, Vol. 292, No. 1, 01.04.2006, p. 142-151.

Research output: Contribution to journalArticle

Maciejowski, J, Ugel, N, Mishra, B, Isopi, M & Hubbard, EJA 2006, 'Quantitative analysis of germline mitosis in adult C. elegans', Developmental Biology, vol. 292, no. 1, pp. 142-151. https://doi.org/10.1016/j.ydbio.2005.12.046
Maciejowski, John ; Ugel, Nadia ; Mishra, Bhubaneswar ; Isopi, Marco ; Hubbard, E. Jane Albert. / Quantitative analysis of germline mitosis in adult C. elegans. In: Developmental Biology. 2006 ; Vol. 292, No. 1. pp. 142-151.
@article{1b897166bd7042c1a112bf0fa3cfaf59,
title = "Quantitative analysis of germline mitosis in adult C. elegans",
abstract = "Certain aspects of the distal gonad of C. elegans are comparable to niche/stem cell systems in other organisms. The distal tip cell (DTC) caps a blind-ended tube; only the distal germ cells maintain proliferation in response to signaling from the DTC via the GLP-1/Notch signaling pathway in the germ line. Fruitful comparison between this system and other stem cell systems is limited by a lack of basic information regarding germ cell division behavior in C. elegans. Here, we explore the spatial pattern of cell division frequency in the adult C. elegans germ line relative to distance from the distal tip. We mapped the positions of actively dividing germline nuclei in over 600 fixed gonad preparations including the wild type and a gain-of-function ligand-responsive GLP-1 receptor mutant with an extended mitotic zone. One particularly surprising observation from these data is that the frequency of cell divisions is lower in distal-most cells-cells that directly contact the distal tip cell body-relative to cells further proximal, a difference that persists in the gain-of-function GLP-1 mutant. These results suggest that cell division frequency in the distal-most cells may be suppressed or otherwise controlled in a complex manner. Further, our data suggest that the presence of an active cell division influences the probability of observing simultaneous cell divisions in the same gonad arm, and that simultaneous divisions tend to cluster spatially. We speculate that this system behaves similarly to niche/stem cell/transit amplifying cell systems in other organisms.",
keywords = "C. elegans, Germ line, GLP-1, Intensity profile, Mitosis, Spatial statistics, Stem cell",
author = "John Maciejowski and Nadia Ugel and Bhubaneswar Mishra and Marco Isopi and Hubbard, {E. Jane Albert}",
year = "2006",
month = "4",
day = "1",
doi = "10.1016/j.ydbio.2005.12.046",
language = "English (US)",
volume = "292",
pages = "142--151",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Quantitative analysis of germline mitosis in adult C. elegans

AU - Maciejowski, John

AU - Ugel, Nadia

AU - Mishra, Bhubaneswar

AU - Isopi, Marco

AU - Hubbard, E. Jane Albert

PY - 2006/4/1

Y1 - 2006/4/1

N2 - Certain aspects of the distal gonad of C. elegans are comparable to niche/stem cell systems in other organisms. The distal tip cell (DTC) caps a blind-ended tube; only the distal germ cells maintain proliferation in response to signaling from the DTC via the GLP-1/Notch signaling pathway in the germ line. Fruitful comparison between this system and other stem cell systems is limited by a lack of basic information regarding germ cell division behavior in C. elegans. Here, we explore the spatial pattern of cell division frequency in the adult C. elegans germ line relative to distance from the distal tip. We mapped the positions of actively dividing germline nuclei in over 600 fixed gonad preparations including the wild type and a gain-of-function ligand-responsive GLP-1 receptor mutant with an extended mitotic zone. One particularly surprising observation from these data is that the frequency of cell divisions is lower in distal-most cells-cells that directly contact the distal tip cell body-relative to cells further proximal, a difference that persists in the gain-of-function GLP-1 mutant. These results suggest that cell division frequency in the distal-most cells may be suppressed or otherwise controlled in a complex manner. Further, our data suggest that the presence of an active cell division influences the probability of observing simultaneous cell divisions in the same gonad arm, and that simultaneous divisions tend to cluster spatially. We speculate that this system behaves similarly to niche/stem cell/transit amplifying cell systems in other organisms.

AB - Certain aspects of the distal gonad of C. elegans are comparable to niche/stem cell systems in other organisms. The distal tip cell (DTC) caps a blind-ended tube; only the distal germ cells maintain proliferation in response to signaling from the DTC via the GLP-1/Notch signaling pathway in the germ line. Fruitful comparison between this system and other stem cell systems is limited by a lack of basic information regarding germ cell division behavior in C. elegans. Here, we explore the spatial pattern of cell division frequency in the adult C. elegans germ line relative to distance from the distal tip. We mapped the positions of actively dividing germline nuclei in over 600 fixed gonad preparations including the wild type and a gain-of-function ligand-responsive GLP-1 receptor mutant with an extended mitotic zone. One particularly surprising observation from these data is that the frequency of cell divisions is lower in distal-most cells-cells that directly contact the distal tip cell body-relative to cells further proximal, a difference that persists in the gain-of-function GLP-1 mutant. These results suggest that cell division frequency in the distal-most cells may be suppressed or otherwise controlled in a complex manner. Further, our data suggest that the presence of an active cell division influences the probability of observing simultaneous cell divisions in the same gonad arm, and that simultaneous divisions tend to cluster spatially. We speculate that this system behaves similarly to niche/stem cell/transit amplifying cell systems in other organisms.

KW - C. elegans

KW - Germ line

KW - GLP-1

KW - Intensity profile

KW - Mitosis

KW - Spatial statistics

KW - Stem cell

UR - http://www.scopus.com/inward/record.url?scp=33646453322&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646453322&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2005.12.046

DO - 10.1016/j.ydbio.2005.12.046

M3 - Article

VL - 292

SP - 142

EP - 151

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -