Quantitation of catecholamines in uninflamed human dental pulp tissues by high-performance liquid chromatography

Richard E. Walton, Mark A. Schachman, Paul A. Rosenberg, Harald A B Linke

Research output: Contribution to journalArticle

Abstract

The quantitation of catecholamines has yet to be reported in the human dental pulp. Various methods of chemical detection have shown the presence of catecholamines in pulpal tissue. Pulpal tissue was obtained from nonrestored, uninflamed teeth that needed to be extracted. The samples were collected, extracted, and analyzed by means of high-performance liquid chromatography with ultraviolet detection for levels of catecholamines (dopamine, epinephrine, and norepinephrine). As mediators of vasoconstriction, catecholamines play an important role in the control of intrapulpal pressure. This research describes a method for quantitation of catecholamines. Future studies investigating inflamed tissue can now be accomplished with the use of this analytic method. The results from this research indicate a baseline level of catecholamines in the uninflamed human dental pulp.

Original languageEnglish (US)
Pages (from-to)83-86
Number of pages4
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Volume80
Issue number1
DOIs
StatePublished - 1995

Fingerprint

Dental Pulp
Catecholamines
High Pressure Liquid Chromatography
Vasoconstriction
Research
Epinephrine
Dopamine
Norepinephrine
Tooth
Pressure

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Radiology Nuclear Medicine and imaging
  • Surgery
  • Dentistry(all)

Cite this

Quantitation of catecholamines in uninflamed human dental pulp tissues by high-performance liquid chromatography. / Walton, Richard E.; Schachman, Mark A.; Rosenberg, Paul A.; Linke, Harald A B.

In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, Vol. 80, No. 1, 1995, p. 83-86.

Research output: Contribution to journalArticle

@article{8eeab4dfbe5145c4b6bcda99d57af841,
title = "Quantitation of catecholamines in uninflamed human dental pulp tissues by high-performance liquid chromatography",
abstract = "The quantitation of catecholamines has yet to be reported in the human dental pulp. Various methods of chemical detection have shown the presence of catecholamines in pulpal tissue. Pulpal tissue was obtained from nonrestored, uninflamed teeth that needed to be extracted. The samples were collected, extracted, and analyzed by means of high-performance liquid chromatography with ultraviolet detection for levels of catecholamines (dopamine, epinephrine, and norepinephrine). As mediators of vasoconstriction, catecholamines play an important role in the control of intrapulpal pressure. This research describes a method for quantitation of catecholamines. Future studies investigating inflamed tissue can now be accomplished with the use of this analytic method. The results from this research indicate a baseline level of catecholamines in the uninflamed human dental pulp.",
author = "Walton, {Richard E.} and Schachman, {Mark A.} and Rosenberg, {Paul A.} and Linke, {Harald A B}",
year = "1995",
doi = "10.1016/S1079-2104(95)80021-2",
language = "English (US)",
volume = "80",
pages = "83--86",
journal = "Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology",
issn = "2212-4403",
publisher = "Elsevier USA",
number = "1",

}

TY - JOUR

T1 - Quantitation of catecholamines in uninflamed human dental pulp tissues by high-performance liquid chromatography

AU - Walton, Richard E.

AU - Schachman, Mark A.

AU - Rosenberg, Paul A.

AU - Linke, Harald A B

PY - 1995

Y1 - 1995

N2 - The quantitation of catecholamines has yet to be reported in the human dental pulp. Various methods of chemical detection have shown the presence of catecholamines in pulpal tissue. Pulpal tissue was obtained from nonrestored, uninflamed teeth that needed to be extracted. The samples were collected, extracted, and analyzed by means of high-performance liquid chromatography with ultraviolet detection for levels of catecholamines (dopamine, epinephrine, and norepinephrine). As mediators of vasoconstriction, catecholamines play an important role in the control of intrapulpal pressure. This research describes a method for quantitation of catecholamines. Future studies investigating inflamed tissue can now be accomplished with the use of this analytic method. The results from this research indicate a baseline level of catecholamines in the uninflamed human dental pulp.

AB - The quantitation of catecholamines has yet to be reported in the human dental pulp. Various methods of chemical detection have shown the presence of catecholamines in pulpal tissue. Pulpal tissue was obtained from nonrestored, uninflamed teeth that needed to be extracted. The samples were collected, extracted, and analyzed by means of high-performance liquid chromatography with ultraviolet detection for levels of catecholamines (dopamine, epinephrine, and norepinephrine). As mediators of vasoconstriction, catecholamines play an important role in the control of intrapulpal pressure. This research describes a method for quantitation of catecholamines. Future studies investigating inflamed tissue can now be accomplished with the use of this analytic method. The results from this research indicate a baseline level of catecholamines in the uninflamed human dental pulp.

UR - http://www.scopus.com/inward/record.url?scp=0029337781&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029337781&partnerID=8YFLogxK

U2 - 10.1016/S1079-2104(95)80021-2

DO - 10.1016/S1079-2104(95)80021-2

M3 - Article

VL - 80

SP - 83

EP - 86

JO - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

JF - Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

SN - 2212-4403

IS - 1

ER -