Quantifying influenza virus diversity and transmission in humans

Leo L M Poon, Timothy Song, Roni Rosenfeld, Xudong Lin, Matthew B. Rogers, Bin Zhou, Robert Sebra, Rebecca A. Halpin, Yi Guan, Alan Twaddle, Jay V. DePasse, Timothy B. Stockwell, David E. Wentworth, Edward C. Holmes, Benjamin Greenbaum, Joseph S M Peiris, Benjamin J. Cowling, Elodie Ghedin

Research output: Contribution to journalArticle

Abstract

Influenza A virus is characterized by high genetic diversity. However, most of what is known about influenza evolution has come from consensus sequences sampled at the epidemiological scale that only represent the dominant virus lineage within each infected host. Less is known about the extent of within-host virus diversity and what proportion of this diversity is transmitted between individuals. To characterize virus variants that achieve sustainable transmission in new hosts, we examined within-host virus genetic diversity in household donor-recipient pairs from the first wave of the 2009 H1N1 pandemic when seasonal H3N2 was co-circulating. Although the same variants were found in multiple members of the community, the relative frequencies of variants fluctuated, with patterns of genetic variation more similar within than between households. We estimated the effective population size of influenza A virus across donor-recipient pairs to be approximately 100-200 contributing members, which enabled the transmission of multiple lineages, including antigenic variants.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalNature Genetics
Volume48
Issue number2
DOIs
StatePublished - Feb 1 2016

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Orthomyxoviridae
Viruses
Influenza A virus
Consensus Sequence
Pandemics
Population Density
Human Influenza

ASJC Scopus subject areas

  • Genetics

Cite this

Poon, L. L. M., Song, T., Rosenfeld, R., Lin, X., Rogers, M. B., Zhou, B., ... Ghedin, E. (2016). Quantifying influenza virus diversity and transmission in humans. Nature Genetics, 48(2), 195-200. https://doi.org/10.1038/ng.3479

Quantifying influenza virus diversity and transmission in humans. / Poon, Leo L M; Song, Timothy; Rosenfeld, Roni; Lin, Xudong; Rogers, Matthew B.; Zhou, Bin; Sebra, Robert; Halpin, Rebecca A.; Guan, Yi; Twaddle, Alan; DePasse, Jay V.; Stockwell, Timothy B.; Wentworth, David E.; Holmes, Edward C.; Greenbaum, Benjamin; Peiris, Joseph S M; Cowling, Benjamin J.; Ghedin, Elodie.

In: Nature Genetics, Vol. 48, No. 2, 01.02.2016, p. 195-200.

Research output: Contribution to journalArticle

Poon, LLM, Song, T, Rosenfeld, R, Lin, X, Rogers, MB, Zhou, B, Sebra, R, Halpin, RA, Guan, Y, Twaddle, A, DePasse, JV, Stockwell, TB, Wentworth, DE, Holmes, EC, Greenbaum, B, Peiris, JSM, Cowling, BJ & Ghedin, E 2016, 'Quantifying influenza virus diversity and transmission in humans', Nature Genetics, vol. 48, no. 2, pp. 195-200. https://doi.org/10.1038/ng.3479
Poon LLM, Song T, Rosenfeld R, Lin X, Rogers MB, Zhou B et al. Quantifying influenza virus diversity and transmission in humans. Nature Genetics. 2016 Feb 1;48(2):195-200. https://doi.org/10.1038/ng.3479
Poon, Leo L M ; Song, Timothy ; Rosenfeld, Roni ; Lin, Xudong ; Rogers, Matthew B. ; Zhou, Bin ; Sebra, Robert ; Halpin, Rebecca A. ; Guan, Yi ; Twaddle, Alan ; DePasse, Jay V. ; Stockwell, Timothy B. ; Wentworth, David E. ; Holmes, Edward C. ; Greenbaum, Benjamin ; Peiris, Joseph S M ; Cowling, Benjamin J. ; Ghedin, Elodie. / Quantifying influenza virus diversity and transmission in humans. In: Nature Genetics. 2016 ; Vol. 48, No. 2. pp. 195-200.
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