Abstract
Genome-wide studies in Ciona often require highly purified cell populations. In this methods chapter, we introduce multi-channel combinatorial fluorescence activated cells sorting (FACS) and magnetic-activated cell sorting (MACS) as two sensitive and efficient tools for isolating lineage-specific cell populations from dissociated Ciona embryos and larvae. We present isolation of trunk ventral cell (TVC) progeny as the test case most commonly used in our laboratory. These approaches may also be applied to purify other cell populations with the proper combination of tissue-specific reporters.
| Original language | English (US) |
|---|---|
| Title of host publication | Advances in Experimental Medicine and Biology |
| Publisher | Springer New York LLC |
| Pages | 101-107 |
| Number of pages | 7 |
| DOIs | |
| State | Published - Jan 1 2018 |
Publication series
| Name | Advances in Experimental Medicine and Biology |
|---|---|
| Volume | 1029 |
| ISSN (Print) | 0065-2598 |
| ISSN (Electronic) | 2214-8019 |
Keywords
- Cell dissociation
- Cell-lineage specific population
- MACS
- Mosaic expression
- Multi-channel combinatorial FACS
- RNA extraction
- TVC
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
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Cite this
Wang, W., Racioppi, C., Gravez, B., & Christiaen, L. (2018). Purification of fluorescent labeled cells from dissociated Ciona embryos. In Advances in Experimental Medicine and Biology (pp. 101-107). (Advances in Experimental Medicine and Biology; Vol. 1029). Springer New York LLC. https://doi.org/10.1007/978-981-10-7545-2_9