Purification of fluorescent labeled cells from dissociated Ciona embryos

Wei Wang, Claudia Racioppi, Basile Gravez, Lionel Christiaen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Genome-wide studies in Ciona often require highly purified cell populations. In this methods chapter, we introduce multi-channel combinatorial fluorescence activated cells sorting (FACS) and magnetic-activated cell sorting (MACS) as two sensitive and efficient tools for isolating lineage-specific cell populations from dissociated Ciona embryos and larvae. We present isolation of trunk ventral cell (TVC) progeny as the test case most commonly used in our laboratory. These approaches may also be applied to purify other cell populations with the proper combination of tissue-specific reporters.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York LLC
Pages101-107
Number of pages7
DOIs
StatePublished - Jan 1 2018

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1029
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Fingerprint

Purification
Embryonic Structures
Cells
Sorting
Population
Larva
Flow Cytometry
Genes
Fluorescence
Genome
Tissue

Keywords

  • Cell dissociation
  • Cell-lineage specific population
  • MACS
  • Mosaic expression
  • Multi-channel combinatorial FACS
  • RNA extraction
  • TVC

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Wang, W., Racioppi, C., Gravez, B., & Christiaen, L. (2018). Purification of fluorescent labeled cells from dissociated Ciona embryos. In Advances in Experimental Medicine and Biology (pp. 101-107). (Advances in Experimental Medicine and Biology; Vol. 1029). Springer New York LLC. https://doi.org/10.1007/978-981-10-7545-2_9

Purification of fluorescent labeled cells from dissociated Ciona embryos. / Wang, Wei; Racioppi, Claudia; Gravez, Basile; Christiaen, Lionel.

Advances in Experimental Medicine and Biology. Springer New York LLC, 2018. p. 101-107 (Advances in Experimental Medicine and Biology; Vol. 1029).

Research output: Chapter in Book/Report/Conference proceedingChapter

Wang, W, Racioppi, C, Gravez, B & Christiaen, L 2018, Purification of fluorescent labeled cells from dissociated Ciona embryos. in Advances in Experimental Medicine and Biology. Advances in Experimental Medicine and Biology, vol. 1029, Springer New York LLC, pp. 101-107. https://doi.org/10.1007/978-981-10-7545-2_9
Wang W, Racioppi C, Gravez B, Christiaen L. Purification of fluorescent labeled cells from dissociated Ciona embryos. In Advances in Experimental Medicine and Biology. Springer New York LLC. 2018. p. 101-107. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-981-10-7545-2_9
Wang, Wei ; Racioppi, Claudia ; Gravez, Basile ; Christiaen, Lionel. / Purification of fluorescent labeled cells from dissociated Ciona embryos. Advances in Experimental Medicine and Biology. Springer New York LLC, 2018. pp. 101-107 (Advances in Experimental Medicine and Biology).
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