Abstract
Genome-wide studies in Ciona often require highly purified cell populations. In this methods chapter, we introduce multi-channel combinatorial fluorescence activated cells sorting (FACS) and magnetic-activated cell sorting (MACS) as two sensitive and efficient tools for isolating lineage-specific cell populations from dissociated Ciona embryos and larvae. We present isolation of trunk ventral cell (TVC) progeny as the test case most commonly used in our laboratory. These approaches may also be applied to purify other cell populations with the proper combination of tissue-specific reporters.
| Original language | English (US) |
|---|---|
| Title of host publication | Advances in Experimental Medicine and Biology |
| Publisher | Springer New York LLC |
| Pages | 101-107 |
| Number of pages | 7 |
| DOIs | |
| State | Published - Jan 1 2018 |
Publication series
| Name | Advances in Experimental Medicine and Biology |
|---|---|
| Volume | 1029 |
| ISSN (Print) | 0065-2598 |
| ISSN (Electronic) | 2214-8019 |
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Keywords
- Cell dissociation
- Cell-lineage specific population
- MACS
- Mosaic expression
- Multi-channel combinatorial FACS
- RNA extraction
- TVC
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Purification of fluorescent labeled cells from dissociated Ciona embryos. / Wang, Wei; Racioppi, Claudia; Gravez, Basile; Christiaen, Lionel.
Advances in Experimental Medicine and Biology. Springer New York LLC, 2018. p. 101-107 (Advances in Experimental Medicine and Biology; Vol. 1029).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Purification of fluorescent labeled cells from dissociated Ciona embryos
AU - Wang, Wei
AU - Racioppi, Claudia
AU - Gravez, Basile
AU - Christiaen, Lionel
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Genome-wide studies in Ciona often require highly purified cell populations. In this methods chapter, we introduce multi-channel combinatorial fluorescence activated cells sorting (FACS) and magnetic-activated cell sorting (MACS) as two sensitive and efficient tools for isolating lineage-specific cell populations from dissociated Ciona embryos and larvae. We present isolation of trunk ventral cell (TVC) progeny as the test case most commonly used in our laboratory. These approaches may also be applied to purify other cell populations with the proper combination of tissue-specific reporters.
AB - Genome-wide studies in Ciona often require highly purified cell populations. In this methods chapter, we introduce multi-channel combinatorial fluorescence activated cells sorting (FACS) and magnetic-activated cell sorting (MACS) as two sensitive and efficient tools for isolating lineage-specific cell populations from dissociated Ciona embryos and larvae. We present isolation of trunk ventral cell (TVC) progeny as the test case most commonly used in our laboratory. These approaches may also be applied to purify other cell populations with the proper combination of tissue-specific reporters.
KW - Cell dissociation
KW - Cell-lineage specific population
KW - MACS
KW - Mosaic expression
KW - Multi-channel combinatorial FACS
KW - RNA extraction
KW - TVC
UR - http://www.scopus.com/inward/record.url?scp=85044230810&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85044230810&partnerID=8YFLogxK
U2 - 10.1007/978-981-10-7545-2_9
DO - 10.1007/978-981-10-7545-2_9
M3 - Chapter
T3 - Advances in Experimental Medicine and Biology
SP - 101
EP - 107
BT - Advances in Experimental Medicine and Biology
PB - Springer New York LLC
ER -