Pulmonary type II cell lamellar body ultrastructure preserved by rapid freezing and freeze drying

J. A. McAteer, Louis Terracio

Research output: Contribution to journalArticle

Abstract

Lamellar body ultrastructure was examined in cultured type II alveolar epithelial cells processed by a method of rapid freezing and freeze drying in the absence of both chemical fixation and solvent dehydration. This method of specimen preparation was chosen to optimize the retention of soluble substances within the type II cell. The use of cultured cell aggregates, in which type II cells line the free surface, facilitated the effectiveness of rapid freezing for the preservation of lamellar body fine structure. Lamellar bodies of frozen/frozen dried type II cells showed none of the often profound lipid extraction artifact produced by conventional processing. Instead, they exhibited a substructure with noteworthy characteristics in common with lamellar bodies processed by resin dehydration lipid retention methods (Stratton, 1976). Importantly, the lamellae of frozen/frozen dried lamellar bodies were contiguous, with no interlamellar space, as is commonly observed in solvent-processed (extracted) specimens. The dimensions of lamellar components in frozen/frozen dried lamellar bodies were, however, different from published values for resin-dehydrated lipid-retained specimens. Lamellar width and the widths of component phospholipid head and fatty acid tail regions in frozen/frozen dried lamellar bodies were approximately 35% smaller than values reported for resin-dehydrated lamellar bodies. This difference was attributed to shrinkage of lamellar components as water was removed from the unfixed tissue during the freeze-drying process. Lamellar bodies preserved by rapid freezing/freeze drying to optimize the in situ retention of intracellular components possess closely adherent concentric membranous lamellae. This supports the contention that the widely appreciated lamellar pattern of the pulmonary lamellar body represents the in vivo molecular organization of intracellular surfactant phospholipids.

Original languageEnglish (US)
Pages (from-to)355-362
Number of pages8
JournalAnatomical Record
Volume209
Issue number3
StatePublished - 1984

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keratinosomes
freeze drying
Freeze Drying
ultrastructure
Freezing
freezing
resin
lipid
lungs
phospholipid
Lipids
Dehydration
dehydration
Lung
Phospholipids
Alveolar Epithelial Cells
cells
Surface-Active Agents
Artifacts
fixation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Pulmonary type II cell lamellar body ultrastructure preserved by rapid freezing and freeze drying. / McAteer, J. A.; Terracio, Louis.

In: Anatomical Record, Vol. 209, No. 3, 1984, p. 355-362.

Research output: Contribution to journalArticle

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